Contributors to Bacteriology In an unassuming way‚. they moved agar from the kitchen to the lab‚ revolutionizing bacteriology WOLFGANG HESSE T RANSLATED BY D IETER H. M. GR~SCHEL Walther Hesse was a well-known community health physician in the Kingdom of Saxony‚ a student of Max von Pettenkofer‚ the father of hygiene‚ and of Robert Koch‚ the father of medical microbiology. His American wife‚ Fanny Angelina‚ introduced agar-agar to the new science of microbiology. The Hesse Family Walther
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Lab Report - Microbes Aim: To investigate four areas of the school and to find out which of the four have the most microbes. Areas to Sample: 1. Girl’s locker room (Senior school) 2. Girl’s locker room (Elementary school) 3. Boy’s locker room (Senior school) 4. Boy’s locker room (Elementary school) Hypothesis: We predict that the boy’s locker room in the senior school will have the most microbes. First of all‚ there are more people using our locker rooms in the Senior School
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of different concentration of protease. In this experiment‚ a plate of milk agar is needed for comparing the result of each sample. Circular wells were dug on the milk agar plates by the use of cork borer‚ and extracts could be put into the wells by dropper. One must note that milk-agar is white in color due to casein. If protease is present‚ casein will be digested by the enzyme giving a clear zone in the milk-agar plate. The size of the clear zone can help us to determine whether fruits differ
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in partial fulfilment of requirements in Biology 101.1 under Prof. Kimverly Hazel Coronel‚ 1st sem‚ 2013-2014 ABSTRACT The effect of molecular weight on the rate of diffusion was assessed using the agar-water gel test. The agar-water gel set up was composed of a six petri dish of agar-water gel containing two wells. Pinch of potassium permanganate (KMnO4) and methylene blue (C16H18N3SCl) were simultaneously introduced to each dish and measured the distance and its rate. Methylene blue‚ having
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appropriate media using aseptic transfer techniques. The necessity and value of collecting‚ identifying and analyzing is critical for learning how diseases are acquired‚ spread and therefore prevented. Materials Sterile nutrient agar petri dish Wax Pencil Lens paper Sterile blood agar petri dish Igniter Immersion oil Sterile cotton swabs Crystal violet dye Pen Incubator Grams iodine Paper Refrigerator Alcohol acetone Distilled water Safranin dye Inoculating loop Sink Bunsen
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Plant Tissue Culture 151 Chapter 9 Plant Tissue Culture Techniques Lorraine Mineo Department of Biology Lafayette College Easton‚ Pennsylvania 18042 Lorraine Buzas Mineo (B.S.‚ Muhlenberg College; M.A.‚ Duke University) is a lecturer in the Department of Biology‚ Lafayette College‚ and has taught botany since 1978 and supervised the General Biology Laboratories since 1970. Research interests in physiological and forest ecology have culminated in several publications. Other interests include
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Practical guide to detection and identification of Phytophthora Leaf blight A number of Phytophthora species cause leaf blight. These include: P. infestans on potato and tomato; P. palmivora on a large number of tropical fruit species including rubber‚ durian and macadamia; and P. colocasiae on taro. These blights on leaves are first seen as small flecks but within 3-5 days they expand to produce large lesions. Initially‚ infected tissue is water soaked but becomes necrotic (brown or black) in
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CORE BIOLOGY PRACTICALS You will need to know these practicals as the exam board may ask you questions based on them. Below is a summary of each one. Name of practical and independent & dependent variables Effect of caffeine on Daphnia heart rate Independent: caffeine concentration Dependent: heart rate of Daphnia Measuring the content of Vitamin C in fruit juice Independent: fruit juice Dependent: volume of juice required to decolourise 1cm3 of DCPIP The effect of temperature on cell membranes
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materials used in this study were Duranta leaves‚ Fusarium sp.‚ petri dish for the containment where cultures of Fusarium sp. were grown. Ethanol for extracting the Duranta leaves. Erlenmeyer flask for containment of the concentrations. Potato Dextrose Agar (PDA) served as the food of the fungus. Gloves‚ face masks and lab gown were also needed for laboratory safety rules. Preparation of Different Concentrations and Treatments Fresh Duranta leaves were collected for extraction. Leaves of Duranta
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to combat bacteria. Hypothesis If bacteria is exposed to garlic‚ then the garlic will to abolish the Escherichia coli‚ because the Escherichia coli (E. coli) is usually a weak bacteria. Procedure Have three petri dishes prepared with blood agar‚ and three test tubes with 100 milliliters of milk. Label three test tubes‚ “A‚” “B‚” and “C.” With a toothpick‚ add a small amount of the E. coli specimen to tube “B"; shake the test tube to mix throughly. For test tube “C‚” add the same amount
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