methodology
Chapter III
METHODOLOGY
Experimental Design Four treatments were made in this study. These were the following treatments,
Table 1. experimental and control treatments
TREATMENTS
DESCRIPTION
1
100% concentration of Duranta Leaves extract
2
75% Duranta Leaves extract
3
50% of Duranta Leaves extract
4
Mancozeb
Preparation of the materials The materials used in this study were Duranta leaves, Fusarium sp., petri dish for the containment where cultures of Fusarium sp. were grown. Ethanol for extracting the Duranta leaves. Erlenmeyer flask for containment of the concentrations. Potato Dextrose Agar (PDA) served as the food of the fungus. Gloves, face masks and lab gown were also needed for laboratory safety rules.
Preparation of Different Concentrations and Treatments Fresh Duranta leaves were collected for extraction. Leaves of Duranta were washed with running tap water before extraction process. The leaves were soaked in ethanol for 48 hours. The different treatments were prepared and were diluted to each level of concentration. For the 100% concentration, it has a full 5 ml application. In 75% concentration, it has a content of 3.75 ml and in 50%, it has a concentration of 2.5 ml.
Sterilization of Glassware
The glassware were washed with tap water and detergent. It was air-dried and sterilized in the oven at 160 C for two hours at the Science Resource Center Laboratory.
Table 2.The treatments and the amount of Duranta leaf extract after Rotary Evaporation
The treatments were applied to the surface of the petri plates and pure culture of Fusarium sp. where it was grown Together with the planting of the fungi, the treatments are also applied to the plates. The plates was incubated for five (5) days and its zone of inhibition was recorded.
Preparation of Culture medium The powdered medium of Potato Dextrose Agar (PDA) was prepared following the given standards. It was sterilized
METHODOLOGY
Experimental Design Four treatments were made in this study. These were the following treatments,
Table 1. experimental and control treatments
TREATMENTS
DESCRIPTION
1
100% concentration of Duranta Leaves extract
2
75% Duranta Leaves extract
3
50% of Duranta Leaves extract
4
Mancozeb
Preparation of the materials The materials used in this study were Duranta leaves, Fusarium sp., petri dish for the containment where cultures of Fusarium sp. were grown. Ethanol for extracting the Duranta leaves. Erlenmeyer flask for containment of the concentrations. Potato Dextrose Agar (PDA) served as the food of the fungus. Gloves, face masks and lab gown were also needed for laboratory safety rules.
Preparation of Different Concentrations and Treatments Fresh Duranta leaves were collected for extraction. Leaves of Duranta were washed with running tap water before extraction process. The leaves were soaked in ethanol for 48 hours. The different treatments were prepared and were diluted to each level of concentration. For the 100% concentration, it has a full 5 ml application. In 75% concentration, it has a content of 3.75 ml and in 50%, it has a concentration of 2.5 ml.
Sterilization of Glassware
The glassware were washed with tap water and detergent. It was air-dried and sterilized in the oven at 160 C for two hours at the Science Resource Center Laboratory.
Table 2.The treatments and the amount of Duranta leaf extract after Rotary Evaporation
The treatments were applied to the surface of the petri plates and pure culture of Fusarium sp. where it was grown Together with the planting of the fungi, the treatments are also applied to the plates. The plates was incubated for five (5) days and its zone of inhibition was recorded.
Preparation of Culture medium The powdered medium of Potato Dextrose Agar (PDA) was prepared following the given standards. It was sterilized