growth of bacteria on Agar plates Hypothesis: That the bacteria will grow in colonies throughout the agar plates except for the one with the anti-biotic loop because some will fight off the bacteria. Method: (Method taken from prac sheet) Plate 1: Use the swab to cover your entire agar plate in your bacteria. You only need one swab of bacteria but be careful to cover the entire surface of your agar in a layer of bacteria. Carefully place an Antibiotic Mastring on top of the agar (use tweezers) in the
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I. Product Background A. Introduction In this highly luxurious extravagant world‚ food and other beverages has always been one of the things which maintain the mainstream of life. Aside from being one of the basics for survival‚ gatherings are also made perfect by food preparation and stress is now often associated with food. This activities show that food intake nowadays is far different from the conventional times. Furthermore‚ this lavish lifestyle most of the people have results in an
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Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates A Special Problem Isolation and Purification of Lyngbya majuscula on Nutrient-enriched Agar Plates ABSTRACT Lyngbya majuscula samples were obtained from the Phycology Laboratory stock culture of the UPV Institute of Aquaculture. Five (5) mm fragments of these were inoculated into agar plates that used 1% agar concentration enriched with varying concentrations (1.0%‚ 1.5%‚ and 2.0%) of Hughes‚ et
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Blue on Agar Plates at Different Temperatures Abstract: Diffusion is the movement of particles from an area of high concentration to an area of low concentration wherein substances are independent‚ rapid and in random motion which frequently collide with each other in the sides of the container. This study aimed to assess the influence of temperature on the diffusion rate of methylene blue on agar plates. To determine the relationship between the two variables‚ five set-ups of agar plates were prepared
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Microbiology Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated‚ why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time‚ propose is to isolate the unknown bacteria. Therefore‚ the first time to streak on the plate‚ there are million of bacteria on the loop. For that reason‚
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Agar From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Not to be confused with auger or augur. For other uses‚ see Agar (disambiguation). Culinary usage Mizuyōkan - a popular Japanese red bean jelly made from agar. Scientific usage A blood agar plate used to culture bacteria and diagnose infection. Agar or agar-agar is a gelatinous substance derived by boiling[1] a polysaccharide in red algae‚ where it accumulates in the cell walls of agarophyte and serves as the primary structural
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Agar and Media Preparation— Agar plates containing King’s B Agar were often used throughout the experiment to support growth of Pseduomonas fluorescens. A recipe was used that included a mixture of 10g Proteose Peptone #3‚ 1.5g Potassium Phosphate Dibasic (K2HPO4)‚ 30ml 50% Glycerol‚ ~965ml water and 20g agar. The mixture‚ post- autoclave‚ was left to cool and 5ml 1M Magnesium Sulfate (MgSO4) was added and created about 40 plates. King’s B Medium was made using the same procedure as the King’s B
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Aim To measure how different concentrations of mythelin blue affects the rate of diffusion through agar jelly. Hypothesis The diffusion of mythelin blue is directly proportional to its concentration‚ hence as the concentration increases; the rate of diffusion increases too. Controlled Variables Time time was kept constant while testing the diffusion spread of mythelin blue with each concentration. Temperature the experiment was undertaken in room temperature as change in temperature can
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Introduction- The purpose of this experiment is to obtain isolation of individual species of particles from the mixed culture. This is completed through the isolation technique of streak plate. The objective of this experiment is to replicate the technique of streak plate but on a much larger scale. Because it is on a larger scale the particles are able to be visually observed as they are isolated using the streaking technique as the experiment is conducted. The benefits of the streaking technique
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The Role of Agarase in Agar-Degrading Bacteria Abstract Agar-Degrading (agarolytic) Bacteria is physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii‚ regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods‚ optimum conditions and the
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