Agarose Gel Electophoresis of DNA Topoisomers Introduction DNA can exist as different isomers that change the confirmation of the DNA’s structure. DNA can be in a linear confirmation this is a relaxed confirmation as the DNA can rotate about its axis unconstrained. It can also exist as a nicked circle this is also a relaxed confirmation as the DNA strands can again rotate freely with respect to one another. Covalently closed circular DNA or cccDNA exists as a supercoil this is because the covalent
Premium DNA
AGAROSE GEL ELECTROPHORESIS Gel electrophoresis is a widely used technique for the analysis of nucleic acids and proteins. Most every molecular biology research laboratory routinely uses agarose gel electrophoresis for the preparation and analysis of DNA. We will be using agarose gel electrophoresis to determine the presence and size of PCR products. Background: Electrophoresis is a method of separating substances based on the rate of movement while under the influence of an electric field
Premium Polymerase chain reaction Gel electrophoresis Molecular biology
MOLECULAR LABORATORY REPORT BIO 615 Name: NUR LISMA RUHILA BT ALIAS Group: AS201 5A Experiment: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL Group partners: 1) HALIMATUN SAADIAH BT MOHD BUSTAMAM 2) NUR FARHANA BT AHMAD SOPIAN 3) FATIN NUR ASYIQIN BT ABD TALIB 4) UMMU AFIQAH BT HASSAN 5) NABIHAH BT MD NAWAWI Date of experiment: 8th October 2012 Date of submission: 15th October 2012 TITLE: GEL ELECTROPHORESIS OF EXTRACTED DNA 0.5% AGAROSE GEL DATE: 8th OCTOBER
Premium DNA Molecular biology Gel electrophoresis
size using agarose gel electrophoresis in an emulation of DNA fingerprinting. The task‚ which was successfully carried out was to determine whether DNA from suspects A‚ B or C matches the sample of blood found at the murder scene (X). The process of PCR acts in the same way as DNA replication but is restricted to specific DNA samples of interest. By amplifying the necessary DNA sequence‚ this procedure is able to produce a usable DNA sample for agarose gel electrophoresis. Agarose gel electrophoresis
Premium DNA Molecular biology DNA profiling
Agarose gel electrophoresis is a technique used in the laboratory to separate macromolecules such as nucleic acids and proteins. Electrophoresis can take a mixture of macromolecules of different molecular weights‚ shapes‚ and various electrical charges to determine all the various compounds in the mixture and allowing for further purification that can aid in details of individual elements of the mixture being studied. Agarose gel electrophoresis is a very important technique used in the field of
Premium Gel electrophoresis Molecular biology Protein
The two techniques that were used to create a DNA profile in this experiment were PCR and gel electrophoresis. The PCR is used to amplify the several DNA samples and gel electrophoresis is performed to separate the DNA fragments according to their size. [6] In the first part of the experiment‚ PCR amplification of the DNA templates was performed and the products obtained were used to perform gel electrophoresis. The process of PCR allows for the amplification of the DNA samples and the components
Premium DNA Molecular biology Polymerase chain reaction
Title: Principles and Practice of Agarose Gel Electrophorsis Objectives: To detect the size ‚ shape and charge of the each dye solution. Methods: Casting the Agarose Gel In this experiment .8% solution was used. By using a 250ml flask the buffer solution was prepared. Using the equation to make enough solution for the intire lab class the equation had to be multiplyed by five. The contents of this equation were added to the 250ml flask and swirled to evenly distrubute it contents
Premium Electric charge Chemistry Gel electrophoresis
Bernardine Date Due: 02/08/2013 PURISIMA‚ Dio Mark Angelo Date Submitted: 02/08/2013 Experiment No. 9 AGAROSE GEL ELECTROPHORESIS OF DNA Abstract _____________________________________________________________________________ Agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar. Agarose is used widely in gel electrophoresis because it gels at a lower temperature‚ does not contain the inhibitors of virus growth frequently present in agar‚ and has more
Premium DNA Molecular biology Gel electrophoresis
The picture above shows a typical gel electrophoresis set up. The clear container in the center of the picture is called a gel electrophoresis chamber. It contains the agarose gel that will be loaded with genetic material‚ as well as a buffer solution. It is connected to a DC power supply via electrodes. This picture was taken at Paw Print Genetics laboratory in Spokane‚ Washington. Viney and Fenton (1998) defined the term electrophoresis as‚ “the migration of charged particles through a static medium
Premium Electric charge Electromagnetism Electron
Gel Electrophoresis Adventure Intro The final goal of this lab was to successfully measure the size of different samples of DNA by placing each sample into a well in agarose gel and running a current through a charged chamber. The DNA samples will move through the gel towards the positive charge. Ideally‚ the DNA will move and create and sequence of smallest to largest. This lab exposes us to DNA technology. Backround Gel electrophoresis is used to separate macromolecules like DNA or RNA by size or
Premium DNA Molecular biology Protein