TSI test because there is no pink color for Urea test and no black color on the bottom of the tube in TSI test. Then‚ follow three tests results are all positive. That is deep blue color for citrate test‚ clear zone surrounding growth for skim milk agar
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MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the
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Yellow | ColourlessMedia: Pink | ColourlessMedia: Yellow | Pink-RedMedia: Pink | ColourlessMedia: Pink | E. coli grown in EMB agar: As we can see from the table above‚ the streak of E. coli on a plate with EMB agar showed a metallic green sheen where E. coli was present. EMB stain is selective for gram-negative bacteria. It is made using 6:1 Eosin and Methylene Blue. EMB agar is a differential media and inhibits the growth of gram-positive bacteria while also using a colour indicator to tell the difference
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are structures called ascospores. It is these structures‚ ascospores‚ where genetic variation that arises from crossing over is easily seen (Davidson). The organism Sordaria Fimicola is a good example of this process because it is easy to grow on agar plates and because they are easy to be seen when looked at through a microscope (Davidson). There are three strands of Sordaria Fimicola used in this experiment; all were retrieved from an area known as the Evolution Canyon. The Evolution Canyon has two
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same control on both sides of the knob and disinfect each side with different disinfectants. There were intervals of 4 minutes‚ in which we let the bacteria sit‚ and swabbed the bacteria off. Immediately after‚ we inserted the sample on a nutrient agar dish. Finally‚ we incubated the samples for approximately 2
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Aluminum foil Masking tape NA powder PDA powder Pentel pen Stirring rod Casserole Electric stove Pressure cooker/ autoclave Steps in Preparation of Culture Media: 1. Calculate the total amount of media needed for the experiment (15ml for plates‚ 5-7 mL for tubes). 2. Weigh the required amount of powder needed to dissolve in distilled water (based on the manufacturers specification in the container). 3. Dissolve the powder using the stirring rod‚ cover‚ cotton stopper and label.
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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7H2O‚ 0.2; CaCl2‚ 0.55; NH4Cl‚ 0.4; agar‚ 1.5. A stock solution of the dye was prepared and used for all studies. The sample collected was screened for CV dye decolorizing bacterial strains by inoculating 10 ml of wastewater into flask containing 100 ml of MSM broth. The flasks were incubated at 35°C under shaking conditions (120 rpm). After 48 hr of incubation period‚ 1ml of the culture broth was appropriately diluted and plated on MSM-CV dye amended agar plates. The morphologically distinct bacterial
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Aseptic Technique and Streak Plates Purpose The purpose of this exercise is to learn aseptic technique procedures and there importance and also to learn to isolate colonies using the streak plate technique. Introduction Bacteria are inoculated (introduced) and cultured (grown) in the laboratory for test studies to determine their morphology (the shape‚ size‚ arrangement‚ and internal structures) and pathology (ability to cause disease). Inoculation has to be performed without adding other microbes
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Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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