There are several different medical reasons for identifying microorganisms. The reasons may vary from having to know the causative agent of a disease in a patient to be able to treat and care for them properly‚ to knowing the correct microorganism to be used for making certain antibiotics as well as proper dosages‚ down to knowing all microbes associated with consumed foods such as plants and animals in case of an allergen or a contamination outbreak. This analysis was done by utilizing all of
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humans. In humans P.vulgaris is known to cause urinary tract infections and wound infections. P.vulgaris is associated with nosocomial infection‚ and has the ability to degrade urea to ammonia by production of the enzyme urease. McConkey agar contains lactose‚ which P.vulgaris does not ferment. It ferments glucose‚ sucrose‚ galactose‚ glycerol occasionally maltose with gas production‚ but never lactose. P.vulgaris ferments liquefies gelatin‚ casein‚ and blood serum‚ curdling milk
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since one little bit of DNA can affect the entire organism thanks to duplication. 2. E. Coli are ideal organisms for molecular geneticists to manipulate because it can easily be grown in suspension culture in mediums such as Luria broth or on agar. Also‚ E. Coli has a relatively small genome‚ containing only about five million DNA base pairs. By chemically and thermally treating E. coli cells‚ they can artificially be transformed. Naturally‚ these cells do not possess the natural system needed
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------------------------------------------------- Isolation and Culture of Zoosporic Fungi using Baiting Technique ------------------------------------------------- Rillera DP ‚ Talibsao K ‚ Talucod AC and Tan P Department of Biological Sciences‚ College of Science‚ University of Santo Tomas‚ España Street‚ Manila 108 ------------------------------------------------- Zoosporic fungi belonging to class Oomycetes of Kingdom Chromista and Phylum Chytridiomycota of Kingdom Eumycota‚ were
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We will be using a method called Polymerase Chain Reaction (PCR) to amplify the 16S rRNA gene present in all prokaryotic cellular life and also culturing samples onto an agar plate for visual identification. This will allow for the accessibility of comparing the similarity or differences in the DNA sequence‚ which can reveal close/distant connection between various bacterial species in the American River community based
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were present and how widespread and abundant they were. Method: In the experiment‚ there were 3 environments in which agar plates were used to get bacteria and fungi. Environment No.1 was inside the lab with the laboratory air‚ environment No.2 was outside the lab in the open air and environment No.3 was taken from scalp scrapings from the students involved. In environment 1‚ 6 agar plates were produced. The 6 plates were separated into groups of 2. Each group of 2 was exposed for different time periods;
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1. Introduction: The goal of this lab was to demonstrate the microbiology technique of serial dilutions and how they can effectively be used in experiments. E. coli cells were exposed to UV light for various amounts of time in order to asses the effect on growth and thereby mutations since this cell was modified to not have photolyse no uvr genes for DNA repair and thus can only use the SOS response. Each group then diluted the cells accordingly based on their UV exposure time and counted the cells
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coli. There is a difference existed among the results obtained from various methods is because the different uses of the methods. LST Broth Presumptive E. coli – Gas and Fluorescense Presumptive Coliforms – Gas and NO Fluoresense VRBA Agar Fluorescent colonies are presumptive E. coli E. coli Petrifilm Red Colonies and Purple – Total Coliforms Purple Colonies with Gas – Presumptive E. coli Comparison between MPN and E coli Petri film: The use of E.coli petrifilm seems more
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they take up the DNA. These bacteria are then allowed to grow and replicate on agar included vector. Some indicator is also added the vectors to indicate which colonies have inserted the vectors. Experiments E2 and E3 were transfection of vectors and analysis of transfection respectively. The vector being used included an ampicillin gene as its selective gene and a fluorescence gene induced with arabinose. Three agar plates were prepared with three different samples. Sample 1 was a control and included
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Pengantar Sebuah proyek mempunyai dimensi yang sangat kompleks dan dinamis‚ diantaranya adalah waktu‚ mutu dan biaya. Kesalahan dalam mengantisipasi perubahan dan kendala yang muncul dilapangan akan menimbulkan adanya resiko bagi perusahaan. Agar berhasil dalam mengelola proyek secara efektif‚ diperlukan kemampuan dan pengetahuan yang cukup bagi personilnya. Untuk mengelola proyek secara umum dengan efektif‚ diperlukan kemampuan dan pengetahuan yang berdasarkan pada PMBOK (Project Management
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