Discussion: For the Feulgen’s/Schiff’s Stain that tests for DNA‚ the samples that contain DNA will show up under a compound light microscope as a magenta color while samples that do not contain DNA will not show up as magenta. A sample of bacteria from an agar plate was used as a control for the DNA test‚ and it showed up as magenta from the Feulgen stain; this confirms that the test accurately indicates the presence of DNA. The results show that samples ALV-188-23 and ALV-843-20 contain DNA‚ which means
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2010 When we initially started the bacteria antibiotic experiment on February 10‚ 2010 our team selected e-coli and Rifampicin (Rb5) to find out the level of resistance e-coli would build towards this antibiotic. When we first checked our agar dish on February 15‚ 2010 we noticed two circles for the zone of inhibition the small circle was 1.5 cm and the medium circle was 3.00 cm. Two days later when we checked it again the small circle had stayed the same at 1.5 cm but the medium
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Nutritional requirements The composition of medium for the tissue culture is the most important key factor in the successful culture of plant cells. The medium should be accurately defined of inorganic and organic chemical additives so as to provide i) the nutrients for the survival of the plant cells‚ tissues and organs under culture and ii) the optimal physical condition of pH‚ osmotic pressure‚ etc. In the culture of plant cells formulating optimum type of medium favorable for in vitro
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arabinose to show the newly expressed DNA by a visible fluorescent glow‚ and a stable control group is what contributes to this experiments thoroughness. It is predicted that the four agar plates will all yield different forms of growth‚ with different coloration and colony number. It is also predicted that the agar plate media containing the arabinose will grow a green fluorescent color and the (-)pGlo plate with ampicillin will exhibit no growth at all‚ due to the lack of the plasmid that is resistant
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Abstract Spore suspension of Bacillus pumilus was inoculated into universal bottles containing sterile distilled water in water baths at temperatures of 85°C‚ 90°C and 95°C. At specific time intervals‚ a sample was removed and spread on nutrient agar plates. The number of colonies formed was used to determine the D-value and z-value. The D-value for 85°C is 64.1 minutes‚ 25.7 minutes for 90°C and 8.2 minutes for 95°C while the z-value is 11.2°C. Introduction Bacterial endospores are very
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UNIVERSITI TUNKU ABDUL RAHMAN CENTRE FOR FOUNDATION STUDIES FOUNDATION IN SCIENCE MAY 2013 FHSB1214 BIOLOGY I TUTORIAL 3 ENZYMOLGY ESSENTIAL Student’s Guide: At university level‚ the tutor facilitates student learning without spoon-feeding. Therefore‚ you are expected to: • Read your textbook‚ attempt the questions before the tutorial • You may enter the class and sign your attendance after showing your tutor that all tutorial questions have been completed; even if you
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called the exposed and the other half was called foiled protected. The second had four sections labeled- Coconut oil‚ SPF 50‚ Sunglass and Control. Next‚ 0.15 ml of yeast was pipetted onto the two separate agars. Then the disposable L-rod spreader was used to spread the liquid so that the agar surfaces are evenly covered. Then the plates were placed in a dark place for 20 minutes. After the 20 minutes the first plate with the exposed half and foiled half‚ the the foiled half of plate was covered
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BioMi 2911 Spring 2014 RUBRIC FOR DESIGNING YOUR OWN EXPERIMENT: EXPERIMENTAL DESIGN AND MINI-PUBLICATION PART 1: EXPERIMENTAL DESIGN Many studies have shown that students learn much about how science is carried out by designing inquiry-based labs. With this in mind‚ a few times during this semester‚ we will ask you to work with your bench mates to design an experiment that will test a given hypothesis—first‚ about Koch’s Postulates‚ then about tracking bacteria in the environment. As
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more potent‚ it is predicted that all three antibiotics will be effective against E. Faecalis‚ with Ampicillin exhibiting the largest zone of inhibition. Materials Enterococcus Faecalis was taken from a stock sample and was spread across a blood agar plate.
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UNIT 9: VIRUSES‚ VIROIDS‚ & PRIONS Lesson 1 – Characteristics of Viruses Depending on their characteristics‚ viruses may or may not kill the host cell. Viruses are too small to be seen with a light microscope and cannot be cultured outside their hosts. Viruses and Bacteria Compared. | Bacteria | Viruses | | Typical Bacteria | Rickettsias/Chlamydias | | Intracellular Parasite | No | Yes | Yes | Plasma Membrane | Yes | Yes | No | Binary Fission | Yes | Yes | No | Pass through
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