of E. Coli‚ Agar‚ LB and Ampicillin and we expect the transformation efficiency to be zero because of the absence of a plasmid. The experimental group is the same as the control group except the plasmid is included. Thus‚ the only difference between the control and the experimental group is the presence of plasmid. The transformation efficiency was calculated in order to determine the impact the plasmid has on transformation by dividing the total number of colonies growing on the agar plate by the
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Introduction To date‚ the bacterial resistance to antibiotics is one of the most important global health threat introduced by the World Health Organization (WHO) and most annual mortality due to hospital infections occurs because of this challenge (1). According to the US Centers for Disease Control and Prevention (CDC) report‚ carbapenem-resistant Enterobacteriaceae (CRE) are one of the three main antibiotic resistance threats. (2). Enterobacteriaceae are Gram-negative bacilli which cause a wide
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We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae is a facultative anaerobe. When inoculated onto an agar slant‚ K. pneumoniae took the shape of echinulate. When inoculated onto a nutrient agar plate‚ we identified that the growth of K. pneumoniae was round and smooth with a convex elevation.
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type of appearance with rod-shaped bacteria in general‚ whether gram-positive or gram-negative.] Perform catalase test. {Catalase test is positive.} Inoculate test tubes prepared with the following mediums – Triple Sugar Iron agar slant (TSI slant)‚ Bile Esculin Agar slant (BEA tube)‚ a methyl-red Voges-Proskauer tube (MR-VP tube) and a Urease tube. Incubate the inoculated tubes‚ to be read at the following lab session. Observe results of tests inoculated during the last session [Note: I used
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tests‚ and the numerous others that are available‚ one can accurately establish the identity of an unknown bacterium. Materials: 1-Tube containing unknown bacteria‚ crystal violet‚ iodine‚ decolorizing agent‚ Safranin‚ Bunsen burner‚ sheep blood agar plate‚ TSA plate‚ and bacitracin antibiotic disks. Procedures 1: 1.) Obtain a tube with an unknown bacteria and materials necessary to prepare a gram-stain. 2.) First place 5-8 loops of the bacteria onto the slide in a circular area and heat
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Agnese‚ A. M. & Cabrera‚ J. L. (1999). The essential oil of Seneciograveolens chemical composition and antimicrobial activity tests. Journal of Ethnopharmacology‚ 66‚ 91–96. 50. Perez‚ C. Pauli‚ M. &Bazerque‚ P. (1990). An antibiotic assay by the agar-well diffusion method. ActaBiologica Medical of Experiments‚ 15‚ 13–115. 51. Samokyszyn‚ V. M. &Marnett‚ L. J. (1990). Inhibition of liver microsomal lipid peroxidation by 13-cis-retinoic acid. Free Radical Biology Medicine‚ 8(5)‚ 491–496. 52. Maron
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Menjaga Kualitas dan Keaslian Batik ‘Made in Indonesia’ Oleh: Dr. Reza A. Nasution Tanggal 2 Oktober adalah tanggal yang sangat penting bagi bangsa Indonesia. Setahun yang lalu‚ tepatnya pada tanggal 2 Oktober 2009‚ UNESCO menetapkan batik Indonesia sebagai warisan budaya dunia (World Heritage). Pengukuhan ini sangat penting artinya bagi bangsa Indonesia yang saat itu tengah berupaya keras mempertahankan ikon-ikon yang menjadi identitasnya dari pengakuan negara-negara lain. Keputusan UNESCO ini
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a relatively lower molecular weight (MW NH3= 17.03 g/mol) than HCl (MW HCl= 36.46 g/mol) diffused at a faster rate (dave NH3 = 19.35 cm) compared to HCl (dave HCl= 16.18 cm). Another experiment was performed with the use of petri dish containing an agar-water gel with three wells. One drop of each substance (potassium dichromate‚ potassium permanganate‚ and methylene blue) was placed on each respective well. In three-minute interval for 30 minutes‚ potassium permanganate‚ which has the lowest molecular
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Pengujian Efek Antimikroba Pelaksanaan Uji efek natimikroba menggunakan metode difusi cakram Kirby Bauer. Suspensi Escherichia coli yang telah diukur kepadatannya di swab dengan menggunakan kapas lidi steril secraa mrata pada media MHA (Mueller Hinton Agar). Selanjutnya kertas cakram yang sceraa terpisah telah direndam ekstrak saun biji jambu merah dengan konsentrasi diletakkan diatas media. Selanjutnya control positif berupa cakram antibiotic kloramfenikol 30 µg serta control negative berupa kertas
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experiment. The first one I will be talking about is the Eosin Methylene Blue agar or EMB for short. EMB is a selective and differential medium that inhibits the growth of gram-positive‚ but detects and isolate gram-negative enteric bacteria. Mythelene blue inhibits gram-positive. The selective part is used for inhibiting gram-positive and has methylene blue. The differential part is what ferments lactose. MacConkey agar is a selective and differential media used to isolate gram-negative bacteria
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