sterilizing it with 70% ethanol. Then trypticase soy agar (TSA) was poured into six groups of 60 Petri dishes (See Appendix 1). The dishes were labeled based on the antibiotic used and were left to dry and solidify at room temperature. After an hour‚ the dishes were placed in a refrigerator at three degrees Celsius. If there was visible condensation on the agar plate‚ they were flipped to avoid the contact of heat that drove the moisture out of the agar dishes. The contents of the broth culture of E.
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This experiment tested the growth of E.coli with inserted plasmid on an agar plate with Ampicillin. One colony of E.coli resistant to Ampicillin was grown during this experiment. The overall goal of the experiment was to successfully grow E.coli on the agar plate‚ which would show that the plasmid had been effectively inserted into the bacteria’s genes. This experiment helped students understand how plasmids were inserted into bacteria and used in real life situations. It also showed how the bacteria’s
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Lab 11 Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology
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EFFECT OF USED OIL IN Trichoderma harzianum A Thesis Presented to the Faculty‚ Special Science Class Iloilo National High School La Paz‚ Iloilo City In the Partial Fulfillment in the subject Research III Sheree Joie Montomo Polonan January 2013 Chapter 1 Introduction of the Study Chapter 1 is divided into five parts: (1) Background of the Study‚ (2) Statement of the Problem and Hypothesis‚ (3) Significance of the Study‚ (4) Definition of Terms
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QUEENSLAND ACADEMIES FOR SCIENCE‚ MATHEMATICS AND TECHNOLOGY International Baccalaureate Diploma Program Extended Essay in Biology Is Tea-tree Oil effective in controlling and minimising the growth of the Escherichia coli Bacteria? A study of the effectiveness of a Natural Antiseptic Candidate Name: Lois Adrienne Dianne Soriano Dulay Candidate Session Number: 003072-032 Word Count: 3997 Supervisor: Ms Kate Barry Date of Submission: 25th July 2011 Acknowledgments Firstly I would
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In the 2nd phase‚ chitin will then be dissolved in less than 40% of Sodium Hydroxide‚ and heated to 90celcius which will convert the Chitin into Chitosan upon dissolving. The third phase will take place over 3 days. The nutrient broth and nutrient agar will be prepared first. On the 2nd day‚ the bacteria broth will be prepared prior to an overnight culture on the same day using E-Coli and M.Luteus. On the 3rd day‚ the Anti-bacterial well diffusion test will be carried out. Finally‚ we will
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facilities and food products which to be analyzed are swabbed. The swab are diluted in a dilutant such as peptone water or phosphate buffer‚ according to the anticipated amount of contamination and subsequently applied to a growth medium containing agar in a sterile‚ covered plate (David‚ Richard and R. 2004). There are many advantages to the cotton swab method. These include the ease with which any health care
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facilitates the handling of large numbers of bacterial clones for classification on a variety of media. METHODS Replica plating. A frequent chore in bacteriological work is the transfer of isolates from one substrate to other selective or indicator agar media. In place of an inoculating needle‚ one might imagine a device consisting of many needle tips in fixed array‚ so that one operation would substitute for repeated transfers with a single needle. The requirements of this design are met by pile
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and starch agar. OBJECTIVES: distinguish between different bacterial species based on colony morphology on agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special
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Materials: 4 sterile cotton swab‚ sterile water in test tube‚ 4 agar plates and 2 blood agar plates‚ wax pencil and labels. Procedure: Appropriately label the cover of each plate as indicated in lab. 1. Determine 2 sampling sources‚ one from your body and one from the surrounding environment. 2. For the first agar plate‚ for sampling from air‚ remove the lids from the plate and allow it to sit uncovered for 15 minutes. 1. For second agar plate‚ open the “stick” end of the sterile cotton swabs to
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