Effect of Molecular Weight on the Rate of Diffusion of Potassium permanganate (KMNO4)‚ Potassium dichromate (K2Cr2O7) and Methylene blue ¹ ABSTRACT The effect of molecular weight on the rate of diffusion was determined using the Agar-water Gel Test. One drop of potassium permanganate (KMNO4)‚ potassium dichromate (K2Cr2O7) and methylene blue were used. Each substance has a respective molecular weight of 158 g/mol‚ 294g/mol and 374 g/mol. The diameters (in millimeter) were measured
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Project Title: Examination of bacterial content of sea water samples from selected sites in South East England Introduction Over the past few years coastal beaches across the UK has seen lavished with recognition for their cleanliness‚ by sections of the media and various organisarions. Majority of these beaches have been awarded the Blue flag award in recognition for meeting set criteria on various standard including cleanliness‚ water quality‚ environmental management and environmental education
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certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth‚ shaded mulch‚ and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The bacteria were then left to grow on their own in a relatively well shaded area. As a control‚ a cotton swab that had nothing on it was used. It was recognized that different types of bacteria exist in different environments and grow in different
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Materials • 30 petri dishes • Nutrient agar (a culture containing agar used to grow bacteria) • Sterile paper disks • Bacillus subtilis broth • Home-built incubator (a sealed box with a heat pad underneath) • Light box • Penicillin • Streptomycin • Tetracycline • Glutaraldehyde • 5 percent phenol alcohol • Fresh garlic extract Procedures 1. Divide petri dishes into six groups of five. 2. With adult supervision‚ melt the agar medium. 3. When liquid agar cools‚ pour an equal amount into each
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- 3 Petri dishes prepared with agar - 1 disinfected swab - 1 bottle of disinfected water - A piece of filter paper - A hole puncher - 4 test tubes - 1 measuring cylinder - 1 pipette with disposable tips - Tetracycline - Clindamycin - Benzoyl peroxide - 1 beaker of water - P. acne bacteria culture - 1 forcep - 1 digital weighing scale - 1 marker pen 1. Before starting the experiment‚ make sure you clean your work area with Chlorox and wear gloves at all
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Hypothesis: The hypothesis for this particular practical experiment is that‚ as the pH decreases or increases from neutral pH of approximately 7‚ the amount of microbial growth on the agar solution will decrease Materials: Agar Solution Preparation: *hand sanitizer/ hand wash soap *small container *1g of agar powder *0.25g of beef extract stock *60mL of heated water *electronic balance *small spoons (2x) *teaspoons (2x) *0.2M Acetic Acid Solution (pH of 3) *0.2M Sodium Hydroxide Solution
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satisfactory method employed to evaluate the effectiveness of antiseptic agents against selected test organism. In this method‚ sterile filter paper disk is impregnated with an antiseotic and placed on fresh heavily inoculated agar plate of a test organism. Following incubation‚ the agar plate is looked for a zone of inhibition. The presence of a clear zone of inhibition surrounding the disk is indicative of inhibitory (antimicrobial) activity against the organism. This experiment deal with the evaluation
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differed between my group’s nutrient agar and MAC agar plates. In courgette‚ there was moderate growth in nutrient agar while it was heavy in Mac agar. In beef broth‚ there was slight growth in nutrient agar while it was negative in MAC agar. In fresh basil‚ growth was heavy in nutrient agar while it was heavy in MAC agar. Where cottage cheese was the ingredient‚ both plates had heavy growth. For walnut‚ growth was slight in nutrient agar while it was negative in MAC agar. Generally‚ the slight difference
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Diffusion in agar cubes Introduction: All cells are dependent on a processes called diffusion‚ which is the movement of a substance from an area of higher concentration to an area of lower concentration. Cells need to obtain certain essential substances‚ like oxygen‚ for their survival. The rate of diffusion is said to be dependent upon the various factors: Size of molecules that have to diffuse‚ concentration gradient‚ the distance across which diffusion occurs‚ number of pores/openings‚ surface
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alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable‚ 1 Pencil‚ marking‚ 11 Petri dish‚ 60 mm‚ 2 Candles (flame source)‚ 1 Thermometer-in-cardboard-tube‚6 Test Tube(6)‚ 16 x 125 mm in Bubble Bag‚ 1 Test tube holder‚ 1 Test-tube-rack-6x21-mm‚ 1 Pipet Graduated Small (5 mL)‚ 1 Baker’s Yeast Packet – Saccharomyces cerevisiae‚ 1 Agar‚ MRS - 18 mL in Glass Tube‚ 4 Agar‚ Nutrient - 18 mL in Glass Tube‚ 1 Broth
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