Quantification of Microbes in Milk and Water Naomi Hall 10/20/14 BSC 381L H003 Group: Megan Baker‚ Katie Coe and Meagan Williams Abstract Coliforms are gram negative bacteria commonly found in the intestines of birds and mammals and are the most widely utilized indicators of fecal contamination. The purpose of this experiment was to test various water and milk samples for the presence of coliforms‚ specifically fecal coliforms. It was expected that fecal coliforms would be found in all
Premium Bacteria Microbiology Escherichia coli
through fused rings‚ their function is to carry cholesterol‚ phytosterol‚ and hormones. The Biuret Reagent test is a straightforward test to identify lipids. To perform this test‚ one must combine equal amounts of a lipids and Biuret Reagent solution‚ let it set for several minutes before analyzing. Once the mixture has settled into its color‚ take a look‚ the substance should have the blue Biuret Reagent solution settled at the bottom and the lipid floating clearly on
Premium DNA Protein RNA
Gene 501 (2012) 85–88 Contents lists available at SciVerse ScienceDirect Gene journal homepage: www.elsevier.com/locate/gene Short Communication A novel method for whole blood PCR without pretreatment Ritu Sharma a‚⁎‚ Amardeep Singh Virdi b‚ Prabhjeet Singh b a b Department of Biochemistry‚ Government Medical College‚ Amritsar-143001‚ India Department of Biotechnology‚ Guru Nanak Dev University‚ Amritsar-143005‚ India a r t i c l e i n f o Article history: Accepted 22 March 2012 Available
Premium Polymerase chain reaction DNA
Purpose: Unknown mixtures will be separated by means of chromatography in which the mixture will be passed in a solution through a medium leaving behind components of the mixture at different rates‚ therefore‚ different spots on the absorbing substance. This will help determine the identity of unknown mixtures. The spot colors on the strip of filter paper and the Rf values of the unknown samples will be compared to those of known samples. To find the position of the spots on the strip of paper‚ we
Premium Chemistry Chromatography Chemical substance
Reagent/Product Structure Hazards First Aid acetone Causes skin and eye irritations‚ hazardous when ingested or inhaled. The substance is toxic to central nervous system (CNS). The substance may be toxic to kidneys‚ the reproductive system‚ liver‚ skin. Repeated or prolonged exposure to the substance can produce target organs damage. . aniline Hazardous in case of skin contact (irritant‚ permeator)‚ of eye contact (irritant)‚ of ingestion‚ of inhalation. Severe overexposure can result
Premium Central nervous system Respiratory system Nervous system
dry by swirling it. Once this is completed the reactivity of alkyl halides under SN1 conditions was tested. Adding two drops of our product and the other different compounds into different test tubes along with 1mL of ethanolic silver nitrate reagent. We are comparing these by seeing how they react and if there is no reaction after 5 minutes we will place the test tubes in a beaker of water that is heated at a temperature of 70oC-80oC and observe what happens. Experimental Stoichiometry
Premium Stoichiometry Distillation Yield
produce three different products. Dehydration reactions are reactions that involve the leaving of an “-OH” or “-H” group resulting in the formation of a double bond between two carbons. For the experiment we used 2-Methylcyclohexanol as a beginning reagent to dehydrate and possibly form 3-methylcyclohexene‚ 1-methylcyclohexene‚ or methylenecyclohexane. Drierite® (Calcium Sulfate) was used as a drying agent to help break the alcohol and hydrogen groups from the carbons. Concentrated phosphoric acid was
Premium Chemistry Water Laboratory
Reaction Time it is useful for testing general alertness and motor speed‚ and is often sensitive. Ludwig Lange‚ working in Wundt’s Institute at Leipsic in 1886-7 found that the duration of the simple reaction was appreciably different‚ according as the reagent directed his attention (so far as possible) exclusively upon the sense-impression or upon the movement to be made in response to it. Lange accordingly distinguished two types or forms of simple reaction‚ the sensorial and the
Premium Psychology Immanuel Kant Concepts in metaphysics
the 2° bromide compound. This observation matched the expectations that a 2-bromohexane would be the likely result of this experiment. However‚ some errors are inherent to this procedure. This can include some problems in the heat reflux step. Some reagents
Premium Chemistry Water Temperature
1) Chemistry Review Key Terms: empirical knowledge theoretical knowledge law of conservation of mass coefficient chemical amount mole Key Concepts: Write chemical equations when given reactants and products (1.5‚ 1.6) Write balanced chemical equations (2.2‚ 2.3) Interpret balanced chemical equations in terms of chemical amount (in moles) (2.3) Convert between chemical amount and mass (2.4) Classify chemical reactions (2.5‚ 2.6) Predict the solubility of elements and ionic and
Premium Chemistry Chemical element Molecule