salicylic acid) from salicylic acid and acetic anhydride. CO2H OH salicylic acid 2g 0.014 mole O O O CH3 H2SO4 ethyl acetate CO2H O O acetyl salicylic acid acetic acid CH3 O + H3C + H3C OH acetic anhydride 5 mL 0.05 mole The limiting reagent is salicylic acid. The theoretical yield of acetyl salicylic acid is 2.52 g. Physical Data:* mp MW salicylic acid 157-9˚ 138 acetyl salicylic acid 135-6˚ 180 acetic anhydride — 102 acetic acid — 60 sulfuric acid — 98 ethyl acetate — 88 *Data
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solution. We then remove the bag out of the beaker‚ and use two test tube which label BAG‚ BEAKER to perform a Benedict’s test. We place solution in the bag in the BAG tube‚ and solution in the beaker in the BEAKER tube and add a drop of Benedict’s reagent to each tube‚ then heat each test tube and observe a color change in each tube. These experiments show that movement of water of chicken egg cell and Elodea cell is “osmosis” and movement of molecules in dialysis bag and beaker is “diffusion”.
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balanced equation will be looked at and then the theoretical yield will be calculated. In order to calculate the theoretical yield‚ two equations will be used: Will be used for both reactants to determine which reactant is the limiting reagent. After the limiting reagent is used‚ then the theoretical yield will be calculated using: This will give the mass of the Acetaminophen. The actual yield will be available in the experimental data‚ and using this knowledge‚ this equation will be used to calculate
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chemical reaction. Stoichiometry is pretty much the math behind chemistry. Doing stoichiometry can calculate masses‚ moles‚ and percents with a chemical equation. The use of stoichiometry is how we were able to find the limiting reagent in this lab. A limiting reagent is the chemical that will be used up first. Many calculations had to be made throughout this lab. Some of the calculations we made were finding the amount of moles of Sodium Hydroxide and Calcium Chloride from the eight different
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1-propanol‚acetic acid and water. (30:10:10:10) Spray reagent—ninhydrin TS. Procedure—Proceed as directed in thin layer Chromatographic Identification Test for Crude Plant Drugs. Develop the chromatogram in a chamber containing developing solvent system until the solvent front has moved about 15cm from the spot point. Remove the plate from the chromatographic chamber‚ mark the solvent front and allow to dry. Spray with the Spray reagent and heat in an oven at 105˚C for 5 to 10 minutes. Observe
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Kinetics of a Reaction I. List of reagents & products 1. 1.0 M Copper(II) nitrate (Cu(NO3)2‚ 0.10 M Hydrochloric Acid (HCl)‚ 0.010 M Potassium Iodide (KI)‚ 0.040 M Potassium Bromate (KBrO3)‚ 0.0010 M Sodium Thiosulfate (N2S2O3)‚ 2% Starch solution‚ Water (H2O) II. Summary of Procedure. Part 1: Find the Volume of One Drop of Solution 2. Fill pipet with 3ml of distilled water 3. Mass a beaker and record 4. Put 5 drops of water into beaker and record
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There could be several reasons why the primer did not work for this study. Primer inconsistencies usually arise due to incorrect PCR conditions or lack of target sequence in the reaction mix. Usually‚ if the problem was with a reagent‚ preparing new stocks of samples and reagents and then setting up a
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titration is a titration technique where the endpoints of the reaction are located from the enthalpy change between the reagents. In thermometric titration we make use of the fact that reactions in solution are accompanied by temperature changes and thus it is possible to follow the course of the reaction with a thermometer. A reagent of known concentration is added to the reagent of unknown concentration and the enthalpy changes presented on a graph to show the endpoint and maximum and minimum temperatures
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therefore react specifically with the target protein. This is what allows for the recognition of the protein of interest from within the background of other cellular proteins. The bound antibody is then detected on the blot by a secondary reagent. This reagent can be either radiolabeled or coupled to an enzyme. Western blots can be used to search for the presence of certain proteins in specific samples‚ tissues‚ or
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oil Oats and water 20 ml gelatin and water solution 20 ml potato and water mixture 20 ml apple juice and water mixture 20 ml unknown substance #1 20 ml unknown substance #2 Paper towels 600 ml beaker Brown paper Biuret reagent Benedict’s solution Iodine solution Procedure: 1. Place test tubes in the test tube rack. Use the grease pencil to label each test tube based on the substance that will be placed in them. 2. To test for starches‚ fill each test tube with
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