Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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ester is prepared‚ and involves heating a carboxylic acid with an alcohol in the presence of an acid catalyst‚ which is used to expedite the reaction. In this experiment the process was used to synthesis isopentyl alcohol‚ which was the limiting reagent‚ with sulfuric acid and excess of acetic acid. Had equal amounts of acetic acid and isopenthyl alcohol been used‚ approximately only two thirds of each would be converted to acetate. This mixture was heated under reflux for one hour to ensure that
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in order to cut my chances of finding my unknown in half. The Indole production test was recorded as negative. It is observed as negative because after adding the Kovac’s reagent there was no color change. Kovac’s reagent added to the tube reacts with DMABA and with any Indole present and produces a compound that turns the reagent layer pink. No color change for an Indole Production means tryptophan is not broken down into Indole and Pyruvate. After finding out I was negative for Indole‚ looking at
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sides of the beaker‚ and then topped. After the eluting solution has risen to within two centimeters of the top of the filter paper‚ the paper was removed from the beaker‚ dried‚ and was stained with a staining reagent solution containing potassium ferrocyanide and potassium iodide. This reagent forms colored precipitates or reaction products with many cations‚ including all of those used in this experiment. The distance of which the spots moved were determined by calculating the Rf value of each substance
Free Solubility Chemical substance Sodium chloride
INTRODUCTION There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose‚ Indole Production‚ and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous
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viscosity other than short setting time. This rapid formation of geopolymer framework will contribute to the early strength development; however workability performance will be significantly reduced. Therefore‚ with the addition of these chemical reagents to the geopolymer binder‚ it will help in identifying the suitable admixtures and also its dosage in order to increase the compressive strength and setting time
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VOLUMETRIC ANALYSIS 1. In volumetric analysis‚ the volume of a reagent needed to react with analyte is measured. 2. In a titration‚ increments of the reagent solution -the titrant-are added to the analyte until their reaction is complete. 3. The most common titrations are based on: - acid-base - oxidation-reduction - complex formation and - precipitation reactions 1. Methods of determining when the analyte has been consumed include: - detecting a sudden change in
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carbonyls‚ Wittig reagent preparation and Wittig rxn‚ Wolff‚ H-based nucleophile carbonyl reduction‚ imine formation‚ reductive amination (rxn only)‚ ketal/acetal formation‚ dithiane chemistry (rxn only)‚ alpha-bromination of ketones‚ -COOH properties (the trends on HW4)‚ Fischer‚ acidic/basic hydrolysis of esters/amides/nitriles‚ CH2N2‚ acid chloride formation (rxn only)‚ acid chloride rxns with esters and amides. 2 Rxn of carboxyllic acid derivatives w/organometallic reagents‚ Cuprate preparation
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Experiment IV Solubility of Dissolved Oxygen Purpose To demonstrate the effect of partial pressure‚ temperature and salinity on the solubility of dissolved oxygen and to demonstrate the interference of nitrite in dissolved oxygen analysis by the Winkler Method. To demonstrate the use of the oxygen electrode and the difference between activity and concentration. References 1. Mancy‚ K. H.‚ Jaffe‚ T.‚ "Analysis of Dissolved Oxygen in Natural and Waste Water‚" USDHEW Public Health Service
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www.kalvisolai.com 5 Mark Compulsory Problems with Solution 1 +2 CHEMISTRY Q. 70 Compulsory Problems with Solution Problems are solved in easiest way (As per Government Answer Key) www.kalvisolai.com 5 Mark Compulsory Problems with Solution 2 SALIENT FEATURES Dear Students ❆ Q.No: 70 is asked as compulsory problem in Govt Exam. ❆ Two problems to be answered out of four problems. ❆ To simplify the problem‚ hints and expected compounds related to molecular formula‚ general formula are
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