Since it’s first introduction in the year 1983‚ Polymerase Chain Reaction (PCR) has very rapidly become a fundamental tool for improving the health and human life. PCR was developed by Dr. Kary Mullis‚ who was at the time working for Cetus Corporation as a chemist. PCR is the quick and efficient method for making unlimited copies of each and every inch of DNA. It can also be adapted to allow amplification of RNA samples as well as DNA samples from any type of organism. PCR is simplified into a 3-step
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Proteins are made up of long chains of amino acids‚ just a chain of ami. tacids makes up the primary structure. The secondary structure is formed by hydrogen bonds joining the chains in certain places to make an alpha helix or a beta sheet. The tertiary structure is formed by even more folding and joining of the chains to make a globular mass or fibrous mass. An example of this would be a carrier protein. Proteins are needed for many things they are needed in our diet for growth and repair of cells
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erythroprotein (en.wikipedia.org). Erythropoiesis is speeded up when oxygen delivery to the kidneys falls and slows down when there is sufficient oxygen-carrying capacity of blood. 4. Nothing would happen. Type O blood has both anti-A and anti-B antibodies. (webmd.com) 5. A. lymphocyte-major combatant in immune responses. (livestrong.com/white-blood-cells) B. basophil- intensifies the inflammatory reaction. It is involved in hypersensitivity reactions. (livestrong.com/white-blood-cells) C. monocyte-
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BIOS 255 WEEK 5 Lab 5 - Lymphatic System & Disease Resistance 1. Describe lymphatic system functions. The primary functions of the lymphatic system are to drain and return interstitial fluid to the blood to absorb and return lipids from the digestive system to the blood‚ and to filter fluid of pathogens‚ damaged cells‚ cellular‚ and cancerous cells to help protect against invasion. 2. Locate each of the following lymphatic vessels: right lymphatic duct‚ thoracic (left
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Membranes were incubated with peroxidase-conjugated bovine anti-mouse IgG secondary antibody or peroxidase-conjugated goat anti-rabbit IgG (1:2000; Jackson Immuno Research Laboratories‚ West Grove‚ PA‚ USA) for 1 h at room temperature. The levels of β-actin (1:2000;Santa Cruz Biotechnology) and lamin A/C (nuclear fraction 1:500 Sigma-Aldrich
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Cardiovascular System: Blood Laszlo Vass‚ Ed.D. Version 42-0007-00-01 Purpose Explain why you did this lab and what if any safety precautions needed to be followed. For this lab I had to prick my finger‚ then with the bleach solution I had to dab it and then carefully drop the blood on the slide. Once I was finished I had to take the second slide and smear the blood. I had to let the slide dry. Then I had to prick my finger again and make 3 more slides. Then I had to mix the chemical
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The objective of the experiment was to differentiate mammalian C2C12 cells into muscle cells and analyze for presence of myosin light chain. Utilizing Western Blotting techniques and using specific antibodies helped to detect myosin light chain. The hypothesis was that proliferating myoblasts could be induced to initiate the differentiation process by depriving the cells of the necessary growth factors and that the differentiation is characterized by the expression of muscle-specific proteins. The
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overcome this‚ a very common strategy is to use secondary molecules such as antibodies‚ to capture the target protein on the surface. An antibody that recognizes the membrane protein of interest can be immobilized covalently on the surface and subsequently capture the target protein when it is injected. Opposite to covalent immobilization‚ where proteins are immobilized randomly‚ the availability of monoclonal antibodies directed against specific epitopes on the target protein makes it possible to
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Summary and conclusion ¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬ 6.1: The chapter 1 deals with the introduction of zoonotic diseases caused by Leptospira and Brucella and their global incidence. Leptospira is ubiquitous in nature affecting both man and animals‚ while Brucellosis is a neglected‚ reemerging‚ zoonotic‚ endemic and communicable disease. Epidemiologically‚ the risk groups will vary according to the climatic conditions thereby can cause epidemics as well as endemics. They are transmitted to the humans when they
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type is determined by a combination of two antibodies and two antigens; namely antigen A‚ antigen B‚ Anti A‚ and Anti B. The surface antigens A‚ B‚ and Rh are the most important. Blood type A has only antigen A‚ but cannot produce Anti-A antibodies because this will cause a self destruction of their blood. However‚ B type blood can be injected into their systems. Type B has antigen B‚ Type AB has both A & B antigens‚ but do not make ABO antibodies. Because their blood doesn’t discriminate against
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