e incorporated a fusion tag prior to our genes of interest and attached the expressed fusion proteins covalently on microarrays. This enhances the specific binding of the proteins compared to nitrocellulose. Thus‚ it helps to reduce the number of false positives significantly. It enables us to screen for immunogenic proteins in a shorter The correct insert size was determined by Colony PCR. Plasmids from clones containing the correct-sized inserts were isolated and the MCS was sequenced using both
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Usually the immune system make antibodies to attack viruses‚ bacteria and germs. However in myasthenia gravis ‚ the immune system for unknown reason see the acetylcholine as a foreign substances thus produce antibodies to block‚ attack and even destroy ACh. The acetylcholine unable to bind to the muscle thus cannot create muscle action potential to move or to contract muscle
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decreases with age and with repeat exposures to the plant. *Transfusing a person of Type A Blood with Type B Blood- ABO blood type system The ABO system consists of A‚ B‚ AB‚ and O blood types. People with type A have antibodies in the blood against type B. People with type B have antibodies in the blood against type A. People with AB have no anti-A or anti-B
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Seroconversion is antibodies that recognize HIV. Before that‚ the antibody of HIV will show a negative result. However‚ as studies suggest‚ there is no treatment for this disease. Moreover‚ the second stage is clinically asymptomatic stage; this stage lasts for ten years at average. It does not include foremost symptoms; however‚ they may show swollen glands. The antibody will show positive result‚ when the level of HIV in the blood is very low‚ so people are carry the disease and HIV antibody is noticeable
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antigens on their cell surface membranes‚ upon a second infection by poxviruses the B memory cells will carry out the humoral response marking viruses for destruction by phagocytes in the bloodstream by attaching to viral antigens with their specific antibodies and the T memory cells will cause the killer T cells to lyse infected cells. As the vaccinia virus is much less virulent‚ when the vaccine is applied‚ the vaccinia viruses replicate locally within the basal epithelium layer of the skin causing a
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Lab12-Medical Microbiology- Part1– Differential Media/Biochemical Tests‚ Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read
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and more preferred than other invasive methods (11‚ 12) . The IgG antibody level to H. pylori is usually increased and may be a marker for H. pylori infection; also because of its high sensitivity and specificity it has been widely used in epidemiologic studies and many physicians didn’t further diagnose their patients for H. pylori infection after an IgG negative serology (13‚ 14). Although the diagnostic utility of IgG antibodies to H. pylori is well established‚ the usefulness of IgA tests is less
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The lab work including a CBC‚ ANA‚ anti-DNA antibody test‚ and complement series. Patient’s lab results were: ANA: 1:640 (normal: No ANA detected in a titer with a dilution 1:32) Anti-DNA antibody test: Elevated (normal: low or none) Complement assay: Decreased C3 level at 43 mg/ld. (normal: 55–120 mg/dL) and decreased C4 level at 14 mg/dL (normal: 20–50 mg/dL) Red blood
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caregivers. Make sure your baby gets his all doses of his whooping cough vaccine. Pregnant Women need a Whooping cough vaccine. Make sure to get the Tdap shot to protect yourself and your baby. After you get the shot‚ your body will create protective antibodies and pass some of them to your
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Tularemia (Rabbit Fever‚ Deer-fly Fever) Tularemia is an infective disease caused by the gram negative bacteria Francisella tularensi (F. tularensis)‚ which is found in habitats limited to the Northern division. (misc.medscape.com)The disease got the name description in 1911 of a plague like illness found in ground squirrels in Tulare County‚ California‚ by Dr. Edward Francis. This bacteria is usually found in rabbits‚ and can also found in other small animals like rodents‚ muskrats‚ beavers‚ squirrels
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