certain situations. Different samples of bacteria cultures were gathered from different places such as the mouth‚ shaded mulch‚ and from the table top. The samples were collected by using a cotton swab and swiped onto a petri dish filled with nutrient agar. The bacteria were then left to grow on their own in a relatively well shaded area. As a control‚ a cotton swab that had nothing on it was used. It was recognized that different types of bacteria exist in different environments and grow in different
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Materials • 30 petri dishes • Nutrient agar (a culture containing agar used to grow bacteria) • Sterile paper disks • Bacillus subtilis broth • Home-built incubator (a sealed box with a heat pad underneath) • Light box • Penicillin • Streptomycin • Tetracycline • Glutaraldehyde • 5 percent phenol alcohol • Fresh garlic extract Procedures 1. Divide petri dishes into six groups of five. 2. With adult supervision‚ melt the agar medium. 3. When liquid agar cools‚ pour an equal amount into each
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Contributors to Bacteriology In an unassuming way‚. they moved agar from the kitchen to the lab‚ revolutionizing bacteriology WOLFGANG HESSE T RANSLATED BY D IETER H. M. GR~SCHEL Walther Hesse was a well-known community health physician in the Kingdom of Saxony‚ a student of Max von Pettenkofer‚ the father of hygiene‚ and of Robert Koch‚ the father of medical microbiology. His American wife‚ Fanny Angelina‚ introduced agar-agar to the new science of microbiology. The Hesse Family Walther
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- 3 Petri dishes prepared with agar - 1 disinfected swab - 1 bottle of disinfected water - A piece of filter paper - A hole puncher - 4 test tubes - 1 measuring cylinder - 1 pipette with disposable tips - Tetracycline - Clindamycin - Benzoyl peroxide - 1 beaker of water - P. acne bacteria culture - 1 forcep - 1 digital weighing scale - 1 marker pen 1. Before starting the experiment‚ make sure you clean your work area with Chlorox and wear gloves at all
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Duranta leaves‚ Fusarium sp.‚ petri dish for the containment where cultures of Fusarium sp. were grown. Ethanol for extracting the Duranta leaves. Erlenmeyer flask for containment of the concentrations. Potato Dextrose Agar (PDA) served as the food of the fungus. Gloves‚ face masks and lab gown were also needed for laboratory safety rules. Preparation of Different Concentrations and Treatments Fresh Duranta leaves were collected for extraction. Leaves of Duranta were washed with running tap water
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Hypothesis: The hypothesis for this particular practical experiment is that‚ as the pH decreases or increases from neutral pH of approximately 7‚ the amount of microbial growth on the agar solution will decrease Materials: Agar Solution Preparation: *hand sanitizer/ hand wash soap *small container *1g of agar powder *0.25g of beef extract stock *60mL of heated water *electronic balance *small spoons (2x) *teaspoons (2x) *0.2M Acetic Acid Solution (pH of 3) *0.2M Sodium Hydroxide Solution
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satisfactory method employed to evaluate the effectiveness of antiseptic agents against selected test organism. In this method‚ sterile filter paper disk is impregnated with an antiseotic and placed on fresh heavily inoculated agar plate of a test organism. Following incubation‚ the agar plate is looked for a zone of inhibition. The presence of a clear zone of inhibition surrounding the disk is indicative of inhibitory (antimicrobial) activity against the organism. This experiment deal with the evaluation
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alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable‚ 1 Pencil‚ marking‚ 11 Petri dish‚ 60 mm‚ 2 Candles (flame source)‚ 1 Thermometer-in-cardboard-tube‚6 Test Tube(6)‚ 16 x 125 mm in Bubble Bag‚ 1 Test tube holder‚ 1 Test-tube-rack-6x21-mm‚ 1 Pipet Graduated Small (5 mL)‚ 1 Baker’s Yeast Packet – Saccharomyces cerevisiae‚ 1 Agar‚ MRS - 18 mL in Glass Tube‚ 4 Agar‚ Nutrient - 18 mL in Glass Tube‚ 1 Broth
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the science lab. In science‚ we would participate in a lab. One of my favorite labs was the owl pellet dissection. Owl pellets are owl throw up. In owl pellets you can find the bones of animals the owl consumed. In this lab we were to take out the bones we found in the pellet and construct the animal we found inside. Most people‚ like me‚ had a sort of rodent‚ but some people found birds in their pellet. Another one of my favorite things we did in science was the Sharpie tie dye lab in fifth grade
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of different concentration of protease. In this experiment‚ a plate of milk agar is needed for comparing the result of each sample. Circular wells were dug on the milk agar plates by the use of cork borer‚ and extracts could be put into the wells by dropper. One must note that milk-agar is white in color due to casein. If protease is present‚ casein will be digested by the enzyme giving a clear zone in the milk-agar plate. The size of the clear zone can help us to determine whether fruits differ
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