POLYMER SYNTHESIS AND PROPERTIES AIM: The aim of the experiment was to prepare three different polymers‚ namely nylon 66‚ Agar Gel and slime‚ being able to differentiate between the configuration and analysis among the three structures‚ noting the physical characteristics of each polymer and the chemical reactions that occur during the formation of the polymer. Pre- lab questions 1. Is Nylon 66 a step or chain – growth polymer? Define both types of polymerization in your answer. Nylon 66
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Apparatus: • Agar Powder • Distilled Water • McCartney Bottles • Brassica Seeds • Scissors Diagram of Apparatus: Method: • Place some Rapid cycling Brassica seeds onto a damp sponge placed in a plastic tray. Cover with cling film and place in a warm‚ light place to germinate. • When the seeds have germinated‚ they are ready to culture. • Measure out 2.5 g of agar powder and add to 250 cm3 of distilled water. Heat and stir gently until the agar dissolves
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cotton swabs are first made damp using sterilized water. The swabs are rubbed in the following 5 places and then rolled and rubbed in the petri dishes: A: Rub the swab on the pass-code for the main entrance of the school and then roll it over the agar in petri dish A. B: Rub the swab on the door handle in room 3.1 and then
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(Salivary amylase) against temperature” aims to know and observe the enzyme activity of the human saliva. The research only included the use of starch-agar as the medium to observe enzyme activity during the experiment. Five starch-agar plates were prepared and each were labeled 1‚ 2‚ 3‚ 4 and 5 respectively. One mL of saliva were placed in each starch-agar plate which was holed then incubated for 24 hours. The plate labeled 1 was stored with 0ºC. The plate numbered 2 was stored at 15ºC‚ plate 3 at
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only for Penicillium citrinum whereas bark oleoresin has caused complete mycelial zone inhibition for Aspergillus flavus and A. ochraceus along with Aspergillus niger‚ Aspergillus terreus‚ P. citrinum and Penicillium viridicatum at 6 lL. Using agar well diffusion method‚ leaf volatile oil and oleoresin have shown better results in comparison with bark volatile oil‚ oleoresin and commercial bactericide‚ i.e.‚ ampicillin. Gas chromatographic–mass spectroscopy studies on leaf
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(Pearson’s 2011). Mannitol Salt Agar The Mannitol Salt Agar test was used to test only the Unknown Gram-positive bacteria. One colony from the Unknown 13A plate was used to inoculate the Mannitol Salt Agar Plate. After the plate was inoculated‚ it was placed in an incubator at 35ºC for 48 hours. If the agar surrounding the culture changed from red to yellow the test was positive‚ which indicated that the bacterium was capable of fermenting mannitol (Pearson’s 2011). If the agar surrounding the culture did
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Microbiology Exam 1 Name_______________________ 1/30/07 1. (1 pt) Who was the first person to observe bacteria using a microscope? a. Lister b. van Leeuvenhoek c. Pastuer d. Koch 2. (2 pts) Which two of the following contribute to the opportunistic and infectious nature of bacteria? a. flagella b. ability to persist in unfavorable environments c. selectively permeable membranes d. fast growth e. ability to sense chemical gradients f. peptidoglycan 3. (1 pt) Capsules‚ sheaths‚ and
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Purpose The principle of this experiment is to have a thorough perceptive of: • Culture washed and unwashed lettuce on agar plate. • Culture fresh and opened milk with the same expiration dates. • Explain the significance of food safety. • Illustrate foodborne sicknesses.
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ASEPTIC TECHNIQUES AND SOURCES OF MICROBIAL CONTAMINATION. Introduction The spread of infections has come to a point where it has become catastrophic. Aseptic technique is the method used to prevent contamination of infections. It is widely used in hospitals‚ pharmacy‚ and pharmaceutical industries and in laboratories. Different establishments have come up with more ways to improve infection control. In hospitals health care acquired infections are costing the NHS £1 Billion a year and
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aeruginosa was gram-positive and therefore it would be susceptible. That was my hypothesis.We divided antiseptic agar plate into quadrants and divided antibiotics agar plate into six areas. We wrote the organisms’ name‚ date‚ and the temperature on the bottom of the agar plates. For antiseptic‚ our group used one color code for each quadrant. We streaked the swab in three directions on the agar plate and used sterilize forceps to remove filter paper discs from the container‚ and dipped it into solution
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