PRE LAB REPORT Reynaldo Riboul TLC and Column Chromatography October 6‚ 2013 Table of Chemicals: Chemical Hazards Mol. Wt. Density Grams Moles Acetone Flammable‚ Irritant 58.08 g mol−1 0.791 g cm−3 2.0 g 0.0344 Hexane Flammable‚ Irritant‚ Dangerous to Environment 86.18 g mol−1 .6548 g mL−1 9.0 g 0.1044 Fluorene Very toxic to aquatic life with long lasting effects 166.223 g/mol 1.202 g/mL 0.3 g 0.00180 Fluorenone Irritant 180.20 g mol−1 1.13 g/cm3 0.3 g 0.00166
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Measuring the Enthalpy change of combustion Abstract This simple experiment is carried out to show the difference in the enthalpy change of combustion between two fuels‚ hexane and methanol. These fuels are individually weighed before and after used as heat source to heat water in a calorimeter. The result is used to find out amount of heat transferred by the fuel‚ amount of fuel used and the enthalpy change of combustion. Introduction Enthalpy change of combustion is the enthalpy change when
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this experiment‚ an adsorption chromatography column was constructed using silica gel as the adsorbent. A 50:50 mixture of ferrocene and acetylferrocene was then separated using the eluents hexane and tert-butyl methyl ether (TBME). The less polar ferrocene was attracted to the less polar hexane‚ so as the hexane passed through the stationary phase‚ the ferrocene moved with it and the acetylferrocene was left behind. When TBME was passed through the stationary phase‚ the acetylferrocene moved with
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Laboratory Techniques pp. 6-‐9 Report: Part A 1) Results in table form Solvent: Solvent: Solvent: methanol Solid Organic Molecules water hexane (intermediate (highly polar) (nonpolar) polarity) Benzophenone Soluble after O Partially Completely 20 seconds soluble after soluble after without C 60 seconds
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Laboratory #16: Analysis of Grease in Water by Solvent Extraction Experiment Date: 2/19/10 Due Date: 2/26/10 II. Objective The goal of this lab was to determine the amount of impurity‚ in the form of oil‚ in water using the method of solvent extraction‚ also known as liquid-liquid extraction‚ a method of separating two materials with different physical and/or chemical properties. Two methods of evaporation will be used to finally determine the concentration of impurity- one using an explosion-proof
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354-355 Experiment 2 SOLUBILITY 1. Part A. Solubility of Solid Compounds. Use your observations to complete the following table‚ rating each system as soluble‚ insoluble‚ or partially soluble. Organic Compound Benzophenone Water Methyl Alcohol Hexane Malonic acid Biphenyl 2. Considering the polarities of the compound and the solvent and the potential for hydrogen bonding‚ answer the following: a) There should be a difference in your results between the solubilities of biphenyl and
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bit more acetone if it evaporated. Next‚ 2 mL of hexane was added to the centrifuge tube. The tube then had 2 mL of water added to it. This was shaken up and then centrifuged for a couple of minutes so that the layers would separate and to get rid of the emulsion‚ which was the green layer. Once the centrifuging process was completed‚ the bottom layer was removed using a pipet. This layer was the hexane layer. A column was assembled for the hexane layer to travel through. This column was a pipet
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the two compounds more completely. The boiling point (bp) of our unknown compounds was taken from the flat regions of the fractional distillation curve. Our unknown mixture contained hexane (bp 69 ºC) and toluene (bp 110.6 ºC). Analysis via gas chromatography allowed us to determine the relative percentage of hexane and toluene at fractions near the beginning and end of our distillations. Relative percentages have been recorded in the table below‚ and our calculations are shown on page 5. Simple
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the two compounds more completely. The boiling point (bp) of our unknown compounds was taken from the flat regions of the fractional distillation curve. Our unknown mixture contained hexane (bp 69 ºC) and toluene (bp 110.6 ºC). Analysis via gas chromatography allowed us to determine the relative percentage of hexane and toluene at fractions near the beginning and end of our distillations. Relative percentages have been recorded in the table below‚ and our calculations are shown on page 5. Conclusion
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mixture of hexane and acetone was determined to best solvent for this extraction. Knowing this add one mL of the pure hexane and one mL of the pure acetone to the mortar. Grind the contents until the cell walls have been adequately broken. Transfer the mixture to a centrifuge tube to separate the sample into layers‚ to help remove the water. Once the sample is finished spinning extract the organic layer and place in a test tube. TLC: To determine which mixture acetone and hexane is the best
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