Presence of Alu element within PV92 on chromosome 18 in a mixed ethnical population and its connection with humans’ ancestral origins By Shiyu Song Biology 155Q LAB-A: 14:30-17:30 Dr. Jacob April 17th 2013 Abstract The Alu insertion (+) within PV92 on chromosome 16 usually reflects a person’s ancestral origin. This study aimed to find the correlation between the presences of Alu insertion (+) within PV92 on chromosome 16 within a population. We used PCR to detect the Alu insertions
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Agarose Gel Electophoresis of DNA Topoisomers Introduction DNA can exist as different isomers that change the confirmation of the DNA’s structure. DNA can be in a linear confirmation this is a relaxed confirmation as the DNA can rotate about its axis unconstrained. It can also exist as a nicked circle this is also a relaxed confirmation as the DNA strands can again rotate freely with respect to one another. Covalently closed circular DNA or cccDNA exists as a supercoil this is because the covalent
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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BRAIN FINGERPRINTING TECHNOLOGY Mandar Ghate Department Of Computers‚ Padmabhushan Vasantdada Patil Pratisthans College Of Engineering mandarghate4@gmail.com Abstract— Brain fingerprinting is a new computer-based technology to identify the perpetrator of a crime accurately and scientifically by measuring brain-wave responses to crime-relevant words or pictures presented on a computer screen. Brain fingerprinting has proven 100% accurate in over 120 tests‚ including tests on FBI agents‚ tests
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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males and females. Gender determination of a human has important applications in forensic science‚ prenatal diagnosis‚ etc. This can be done by Polymerase Chain Reaction (PCR) followed by gel electrophoresis. The presence of two bands of DNA indicates that the sample is from a male while the presence of one band of DNA indicates that the sample is from a female (sasaki and shimokawa‚ 1995). In this experiment‚ Chelex was used to extract nucleic
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will finish off talking about PCR‚ and then we will discuss how it can be used. If we go back to the slide of the double stranded DNA‚ and if we take that to a high temperature‚ the two strands separate‚ you then add the primers‚ which interact with ? On the strand‚ synthesis takes place in the 5-3 direction‚ then you end up with 2 molecules identical to the DNA‚ and then you do another round‚ so it’s an exponential increase. There are different enzymes and polymerases‚ which come from bacteria
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Hassuneh. Report subject: Serum protein electrophoresis. Report No.: 1. Serum Protein Electrophoresis (SPEP) Introduction: The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases. And its uses an electrical field to separate the proteins in the blood serum into groups of similar size‚ shape‚ and charge. And here we’ll use gel electrophoresis which indicate that blood serum is placed on special paper treated with agarose gel and exposed
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Position Paper #2 Fingerprinting At Birth I think that having everyone fingerprinted at birth is a great idea for many reasons. First off‚ it can help if something was to happen to you and they needed to identify you by your fingerprints. Second‚ it can help more in crimes. Lastly‚ it can help if someone is kidnapped. Now-a-days‚ people are more violent and things happen more frequently than it should. People go missing and turn up dead. Some people are so badly damaged that they cannot be identified
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DNA Barcoding Click on the following link and view the background on DNA barcoding: https://www.dnalc.org/resources/animations/dna-barcoding.html 1. What is a DNA barcode? DNA barcoding is a fast accurate method of identifying plants and animals‚ or products made from them. DNA barcode is DNA sequence that uniquely identifies each species of living things. Short DNA sequences are used to identify species by comparing them with the known barcodes in large databases. When you get to the section where
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