What is gel electrophoresis? Gel electrophoresis is a technique that separates pieces of DNA (or other biological molecules) by size. Pieces of DNA in a test tube all look the same. DNA Gel Electrophoresis Gel electrophoresis separates pieces of DNA by size so that researchers can further analyze them BURST Training Session November 29‚ 2005 Once the DNA samples are loaded onto the gel‚ an electric current is applied to the gel. DNA is negatively charged due to all the phosphate groups in the
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General Biology I‚ Fall 2011 Lab Report 3 Name: Luan Nguyen Date: 11-30-11 Lab: Gel Electrophoresis Purpose of this lab: In this laboratory investigation‚ students will analyze hypothetical human DNA using restriction endonucleases and gel electrophoresis to match samples from a crime scene to a suspect. Introduction: Your analysis unit will use gel electrophoresis to determine the guilty party. Electrophoresis is a technology in science that allows an individual to separate
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The purpose of the DNA extraction lab was not only to inform students on how DNA is present in humans and all organisms‚ but to also educate them on how DNA can be extracted using common household materials. Also‚ the lab was very efficient as it introduced the students to extracting their own DNA found on their cheek cells as well as letting them take an observation on how DNA appears or how it is formed. Additionally‚ students were instructed through a very clear and simple procedure‚ which guided
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this‚ forensic scientists are forced to "bring out the big guns." Researchers can compare DNA samples from bodies to those taken directly from the victim: from hair‚ a toothbrush‚ a family member‚ and etcetera (Whitfield 6). As a result of the terrorist attacks‚ forensic scientists have come up with innovative tests and ways to interpret the resulting identification data (Whitfield 1). According to forensic DNA expert John Butler (National Institute of Standards and Technology in the city of Gathersburg
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Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed
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DNA Lab Report SungYong Jang What is DNA? What do the letters stand for? What is it composed of? Where is it found? What is it shaped like? Answer in full sentences. It is the genetic material that can be passed on from parent to offspring DNA --> Deoxyribonucleic acid It composed of Deoxyribose (5 carbon sugar)‚ a phosphate and a Nitrogonous base (the 4 N-bases are Guanine‚ Cytosine‚ Adenine and Thymine) It is a double helix (looks like
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DNA Extraction of a Musa acuminata Biology Lab Report \Analyze and Interpret A number of steps are required to isolate DNA from cellular contents. Describe what happens at each step‚ and why it acts to separate the parts of the cell. /6 There are three specific steps required to isolate DNA from its cellular contents. The steps used to remove and expose DNA from its cell are: breaking down the food type you are using by crushing it‚ for example a banana or strawberries‚ exposing the substance
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Strawberry DNA lab Extraction Purpose: The purpose of this lab experiment is to see if this particular type of fruit which in this case is a strawberry contains DNA. Using materials such as a Ziploc bag to help keep the entire strawberry in one place while it gets smashed and test tube to help better see the DNA and break down of the liquid in the left over strawberry sediments. Background information: The long thick fibers of DNA store the information for the function of the chemistry
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EXTRACTING AND PURIFYING GENOMIC DNA FROM A RAT LIVER FOR ELECTROPHORESIS Deoxyribonucleic acid (DNA) is a molecule that encodes the genetic instructions used in the development and functioning of all known living organisms and many viruses. Along with RNA and proteins‚ DNA is one of the three major macromolecules essential for all known forms of life. Genomic DNA is the DNA that holds the complete set of genetic data for an organism. In humans‚ the genomic DNA spans 46 chromosomes‚ providing a complete
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Objective: The purpose of this lab is to allow you to become familiar with a procedure for extracting DNA‚ collecting DNA samples‚ and to observe the physical characteristics of DNA Background: The wild strawberry is a diploid because it only has two sets of chromosomes. While the grocery store kind is an octoploidy because it has eight sets of chromosomes. The reason we chose the grocery store strawberry is because we’d be able to extract more DNA. Ripe strawberries make enzymes which help break
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