DNA replication mechanism is an integral and the most important part of cell division. It is a complicated mechanism that requires a lot of steps and different enzymes. Many scientists were trying to understand the mechanism of DNA replication and its role in the transfer of the hereditary information to the offsprings. DNA replication begins with a single origin of replication that starts off the process by creating a replication bubble. In many eukaryotic cells‚ the DNA strand will have more than
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TRANSCRIPTION: Transcription is the process of which DNA matches corresponding RNA bases‚ Transcription is located in the Nucleus‚ and the only type of RNA that is involved in Transcription is mRNA‚ and the purpose is so that the code can get out of the Nucleus‚ mRNA is also made through Transcription‚ It also takes information that doesn’t directly make proteins but it helps makes codes for the production of proteins‚ DNA Transcription consist of 4 nucleotide bases‚ Adenine‚ Thymine‚ Cytosine‚ Guanine
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Title Application of DNA Barcodes to Identify Various Plant Species Abstract In this experiment we applied barcodes to plants in order to identify what species they are classified under. We also compared the DNA sequences of different plant species using the ribulose-biphosphate carboxylase gene (rbcL). We took samples from a plant called Chard and performed PCR‚ DNA amplification and quantification and sequenced the DNA. During the experiment‚ we hypothesized that this year’s “nonspinach”
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The investigation on the average mass of DNA with the mass of banana‚ strawberry and kiwi. Purpose: The purpose of this lab is to investigate the comparison between the amount of DNA per gram of fruit that can be extracted from a banana‚ strawberry and kiwi and to determine which one has more DNA. Hypothesis: The banana genome contains 837 MBPs and the strawberry genome contains 206 MBPs and the kiwi genome contains 128 MBPs. This states that there are more base pairs in a banana genome
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Module 2 Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form
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extract DNA from a strawberry using limited household items. This was accomplished with the prior knowledge that the DNA is stored in the nucleus of the cell‚ and that is an extraction buffer would be needed to acquire the DNA. This experiment had no control group‚ however‚ if there was a control group it might be a group with no extraction buffer. The independent variable in this experiment is the extraction buffer. The dependent variable in this experiment is the ability to extract DNA. The constants
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Gel electrophoresis is a routine laboratory procedure in biochemical studies that takes advantage of a protein’s amphoteric nature to determine its molecular weight and charge by running the sample through a gel matrix under the influence of an electrically charged field. A popular example of gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide electrophoresis or SDS- PAGE which has been used in this experiment to supposedly determine albumin and casein’s molecular weights respectively. The
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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DNA extraction and purification is an essential tool in understanding the biological basis and significance of a eukaryotic cell. Its role is pivotal to numerous scientific applications ranging from basic science research to applied research. Hence‚ DNA is found in all living organisms. Fragaria x ananassa‚ also known as a strawberry has been widely used as one of the many biological models in studying DNA structures due to its accessibility. Strawberries are octoploid which contain large genomes
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Radioisotopic labels would be used in experiments to identify semi-conservative replication in prokaryotes. Because we anticipated that a labeled DNA would have different density with unlabeled‚ which means‚ by analyzing the different density of DNAs‚ we can determine which of DNA is labeled‚ half-labeled or unlabeled. To this end‚ I will use c13 label the bacteria and abruptly change carbon source with C12. Then I will collect four samples in different time and analyze the results from centrifugal
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