CORE BIOLOGY PRACTICALS You will need to know these practicals as the exam board may ask you questions based on them. Below is a summary of each one. Name of practical and independent & dependent variables Effect of caffeine on Daphnia heart rate Independent: caffeine concentration Dependent: heart rate of Daphnia Measuring the content of Vitamin C in fruit juice Independent: fruit juice Dependent: volume of juice required to decolourise 1cm3 of DCPIP The effect of temperature on cell membranes
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beetroot will be heated for the same time for each one‚ also it will be left in the water for the same time after. Equipment:- Beaker Thermometer Heat proof mat Tripod Bunsen Burner Cork Borer 10 Beetroot cylinders Forceps 10 test tubes Colorimeter Measuring cylinder Also to improve the reliability of the test I will repeat the results. Method - Heat 200ml of water in a beaker - Whilst water is heating‚ place 10cm3 of water into each of ten test tubes. Label 85-45 and place in a water
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Experiment 19 - Determination of the equilibrium constant for the reaction Fe3+ (aq) + SCN( (aq) = FeSCN2+ (aq) Object: To determine the equilibrium constant by a colorimetric method Theory: Colorimetric methods of analysis are usually applied to the determination of small concentrations of either inorganic or organic materials in a solution. The constituent sought must be coloured or must be capable of reacting with a reagent to produce a substance having a suitable colour. Beers Law‚ which
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Work Specific Skills Work specific skills focusing on the use of standard operating procedures for different types of laboratory equipment‚ the calibration of standard laboratory equipment and the use of a lab book. This was chosen as a priority for me due to these skills being an essential part of any work I wish to do in the future‚ working or continuing my studies with in a lab. This set of skills is relevant to my skills matrix because it is a skill I have only had the chance to develop at
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determine the accuracy of the copper and zinc composition percentages in a random sampling of pennies. The penny was dissolved to make aqueous copper ions and four copper dilutions were made from stock solution. Each cuvette sample was measured in a colorimeter and the data was plotted linearly using Beer’s law plot. Mass percent and percent error were found using calculations. Analysis of class data provided further data to determine the copper and zinc composition percentages. Experimental Procedure
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hydrous copper sulfate needs to be determined. Clean the glassware then get four 50 mL Erlenmeyer flasks with 25 mL of the stock solution and aqueous solvent. Prepare all the unknown sample solutions for determining Beer’s Law Graph. Turn on colorimeter at 100% T then calibrate. Get the data and analyze it if it is correct. Experiment 3 – Percentage of sulfate in sample. Weight out the sample and dissolve it. Heat it and then slowly add the precipitating agent. Heat it for about 30 minutes while
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activity). Algal bloom is the direct result of eutrophication where suffocation of aquatic life and nutrient depletion may occur. Professionals may use a colorimetric analysis to measure the absorption of light from coloured water sample using a colorimeter to determine the concentration of nitrate and phosphate present. An acid base titration can also convert nitrogen into ammonia to examine the result. It is
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this reaction. Hypothesis: If the reaction between crystal violet and sodium hydroxide reacts appropriately‚ then the order will be first order. Procedure: Mix 10 mL of sodium hydroxide and crystal violet solution together. After calibrating the colorimeter‚ insert the mixture. Absorbance data will be collected for three minutes. Analyze the data graphically to determine the reaction. Discussion: The hypothesis that I stated was sadly‚ not supported by the data that my partner and I obtained. Comparing
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Support Material Exemplar Starter Pages GCE Chemistry B (Salters) OCR Advanced GCE in Chemistry B (Salters): H435 Unit: F336 Chemistry Individual Investigation This Support Material booklet is designed to accompany the OCR Advanced GCE specification in Chemistry B (Salters) for teaching from September 2008. Contents Contents 2 Support Materials 3 Choice of investigation topic 4 Health and Safety 33 Other forms of support 35 Support Materials These support materials should
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which the absorbance is the at the greatest. [pic] INTRODUCTION A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the solution. A spectrophotometer differs from a colorimeter in the manner in which light is separated into its component wavelengths. A spectrophotometer uses a prism to separate light and a colorimeter uses filters. Both are based on a simple design of passing light of a known wavelength through
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