absorbance. As the reaction between the chemicals takes place and the Crystal Violet receives the hydroxide the overall intensity of the purple color will decrease thus affecting the absorbance. The absorbance of the solution will be measured with a colorimeter as the reaction takes place and will be interpreted as a direct representation of concentration of Crystal Violet. After the reaction has taken place‚ through analysis of graphs plotting absorption vs. time‚ the natural log of absorption vs. time
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Apparatus Material and Equipment: 0.00200 M KNCS‚ 0.00200 M Fe(NO3)3 0.200 M Fe(NO3)3‚ 0.10 M HNO3‚ 5 Cuvettes‚ 1 colorimeter 4 100ml beakers‚ 5 Test tubes 2 250ml Graduated cylinder. The first step is to calibrate the colorimeter with0.20 M Fe(NO3)3and set the absorbance at 470 nm since it is known to keep an acidic solution throughout the entirety of the experiment. It was important to do this right at the beginning of the lab since the zeroed value of the acid was the
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An investigation to measure the percentage light transmission‚ using a colorimeter‚ through a solution‚ from pH 2 – pH 9‚ in which jelly cubes were immersed over a 24 hour period Interpretation Written Communication of the Data C1 Pepsin is an enzyme that works in the stomach and has an optimal pH between pH 1 and 4 or in acidic conditions. From our graph it can be seen that that the lowest mean percentage light transmission for pepsin is when the buffer has a pH of 2. Trypsin is an enzyme that
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absorbance of some light wavelengths by solutes dissolved in solution‚ while allowing other wavelengths to pass through (transmittance). The combination of the remaining wavelengths that pass through results in the colour of the solution. A colorimeter can be used to determine the amount of light at a particular wavelength that is absorbed/transmitted by a solution. Depending on the concentration of the solute in the solution‚ more or less absorbance/transmittance will result. A = lc where
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Biology Lab Report - Movement in and out of cells (4A) Shiang Chen Ting (24) Sec 313 Introduction The aim of this experiment is to explore the factors which affect the movement of materials in and out of the cells. The dark red or purple colour of Beetroots is caused by a pigment called betalain that is found within the vacuole of the beetroot cells. The pigment remains intact within the cell if the cells are not “stressed” by the external environment. In this exploration‚ two factors that affect
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Unit Name: Analysis at Work Unit Number: G621 Assignment Title: Finding the Percentage of Copper in a Brass Alloy Assignment: G620 Sample Assignment C Date Set: Due Date: Assessment Objective(s): AO3 Vocational Brief: Brass is an alloy of copper and zinc. This alloy has many uses ranging from coinage to non-ferrous fittings on ships. Many domestic taps use brass where contact with water occurs. You are provided with a sample of brass which is about 150 years old and you are
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MICROBIOLOGY COURSEWORK The effect of antibiotic concentration on bacterial growth Introduction Null Hypothesis- Increasing the antibiotic concentration has no effect on bacterial growth. Experimental Hypothesis- Increasing the antibiotic concentration decreases bacterial growth. Scientific Theory Bacteria are prokaryotes and can be identified by their shape. Spherical bacteria are called cocci‚ rod shaped bacteria are called bacilli‚ spiral shaped bacteria are called spirilla and
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FeSCN2+ Kc = [FeSCN2+]/{[Fe3+][SCN-]} 3) Objectives: To determine the equilibrium constant; Kc of the reaction between Fe3+ and SCN- ions 4) Materials: Computer‚ Vernier computer interface‚ Logger Pro‚ Vernier colorimeter‚ 1plastic cuvette‚5 test tubes of 20x150mm‚ thermometer‚ 0.0020M KSCN‚ 0.002M Fe(NO3)3( IN 1.0M HNO3)‚ 0.2M Fe (NO3)3 (in 1.0M HNO3)‚ 4 pipets‚ pipet bulb‚ tissues. 5) Safety measure: Wear goggle and gloves to protect against
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find the optimum concentration for maximum absorbance. C = A/ ε Methods: The two reagents used are Bromophenol blue and Methyly orange. Experiment 1: 1 Set up the colorimeter at 400nm‚ measure the absorbance of both reagents against distilled water‚ this will be used as a blank (make sure to zero the colorimeter after each interval) 2 Repeat this procedure at 20nm from 420nm to 700nm 3 After getting a range of results make further readings in smaller intervals to precisely locate
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What is the Effect of the Colour of Light on the Rate of Photosynthesis? By Amelia Rowan‚ 12SDa Aim To discover whether the colour of the light source has any effect on the rate of photosynthesis of a sample of algae. Hypothesis It was predicted that the colour of the light source would have a very slight effect on the rate of photosynthesis. It was further predicted that when the sample was placed under the green light‚ the rate of photosynthesis would be the lowest. This hypothesis was arrived
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