to it‚ the solution will turn blue-black. Aim: The aim for this experiment is to investigate how the different buffer solutions work on the enzyme amylase. This will be investigated by using iodine and by timing the experiment using the colorimeter and the program Loggerlite. Hypothesis: The pH denatures enzymes‚ and since amylase is an enzyme‚ my hypothesis is that the pH will change the shape of the active site of the enzyme‚ so the substrate will not be able to fit in anymore.
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An investigation into the effects of temperature on enzyme action An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems‚ for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate
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boiling tube racks Thermometer (one for each water baths) Colorimeter Cuvettes Stopcocks Distilled Water Pipettes Small measuring cylinder Independent variable: Temperature (°C) – this will be controlled by the water baths and each water bath will have a thermometer placed inside. The temperature will range from 0°C to 70°C and will go up in 10. Dependent variable: % of Absorbance- the absorbance will be measured by the colorimeter which will have 2cm3 of the dye solution to make it a
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Practical Assessment Planning AIM Beetroot cells contain a red pigment‚ which is stored in the cell vacuole and a vacuole membrane to prevent this leaking out of the cell surrounds it. The outer of the cell is also surrounded by a membrane‚ which again helps contain the pigment inside the cell. In this experiment I aim to find out the relationship between the leakage of red pigment from a beetroot cell and the surface area. To do this successfully I will need to alter the surface area of the beetroot
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The reaction order‚ based on the rate law‚ was first order with respect to crystal violet and second order with respect to OH-. The rate law was as follows: Rate law = k [CV]1[OH-]1 where k equaled 2.61. In order to determine the reaction order with respect to crystal violet‚ the graph that described the relationship between ln[CV] and time (seconds) was Figure 2. Not only did Figure 2 generate a more linear relationship‚ but it had the highest R2 value of 0.992 than ([CV] versus time) and ([1/CV]
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damaged‚ the red pigment will leak out into the surrounding environment. The intensity of color in the environment should be proportional to the amount of cellular damage sustained by the beet. To measure the color intensity‚ you will be using a Colorimeter or Spectrometer. In this device‚ blue light from the LED light source will pass through the solution and strike a photocell. The alcohol solutions used in this experiment are clear. If the beet pigment leaks into the solution‚ it will color the
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| [Solubility Product Constant] | Experiment 11 | | Molly McCabe | 4/18/2013 | | Data Tables: Part 1 and Part 2 Experimental Data: Standard | Calculated volume of copper sulfate in standards | Absorbance values | Blank | 0.0 mL | 0.0 | 1 | 1.25 mL | 0.094 | 2 | 3.75 mL | 0.065 | 3 | 2.50 mL | 0.099 | 4 | 3.50 mL | 0.129 | 5 | 5.00 mL | 0.187 | Collected Data | Test tube 1 | Test tube 2 | Test tube 3 | Test tube 4 | Test Tube 5 | Initial volume (mL) | 0.05
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Kinetics of the Harcourt-Essen Reaction Name: Manpreet Kaur Candidate Number: 7123 AS and A2 Aims: AS AIMS: 1. Investigate the effect of temperature on the rate of reaction. For this aim 3 sets of results will be obtained by timing how long it takes for the colour change to occur in different temperatures determined by the use of an electric water bath. With these results‚ the effect of temperature on the rate of reaction will be investigated. 2
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order of temperature of the water bath. Describe any relationship between the amount of pigment released from the beetroot and the temperature. g. If you have access to a colorimeter‚ set it to respond to a blue/ green filter (or wavelength of 530 nm) and note whether you are measuring absorbance or transmission. Check the colorimeter reading for distilled
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Aim: This experiment aims to determine what effect an increase in the surrounding temperature has on the plasma membrane of a typical plant cell structure. Hypothesis: An increase in temperature will damage and denature the plasma membrane and cause the cytoplasm and other substances contained within the membrane to leak out. Introduction: The purpose of a cell membrane is to control the transport of substances moving into and out of a cell. The membrane is an extremely thin layer (8 to 10 manometers
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