Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
Premium Cell culture Cell
Title: The effect on temperature on beet cell membranes. Introduction A cell membrane or plasma membrane is very important in protecting cells. A cell membrane is a thin layer that protects the cells. The membrane is filled with lipids and proteins. The cell membrane protects the cell from ions and molecules that enter and exit the cells. I hypothesize that at -5 degrees Celsius‚ the color intensity will be a 1. I hypothesize that at 5 degrees Celsius‚ the color intensity will be a 2
Premium Temperature Cell membrane Protein
1) A peptide‚ KKDSRHSTTR is tightly bound to a negatively charged ion exchange column. The column is washed with a pH 7.2 HEPES buffer (50 mM) and the peptide does not elute. Suggest two ways that you can change the buffer that will make the peptide elute from the column. 2) What amino acid(s) is/are involved in crosslinking polypeptide chains. How can these crosslinks be cleaved and prevented from reforming? 3) A polypeptide is subjected to the following degradative techniques results
Premium Protein Amino acid
Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment in boiling
Premium Molecular biology DNA
Biochemistry of Photosynthesis Overall Word Equation: Water+ Carbon Dioxide( Carbohydrates+ Oxygen ((: light + chlorophyll) 1. Light Dependent Stage/ photochemical reaction/ light reactions: Word Equation: Water ( Hydrogen ions + Oxygen ((: light + chlorophyll) Site: On the Thylakoid membrane of the chloroplast Major events: • Light absorption o Chlorophyll in the thylakoids absorbs light. The
Free Photosynthesis
John Leone Lab Partner(s) Ethan Davis & Ryan Hanna Date: 12/10/14 Instructor: Dr. Chuckran Period: H Plants and Animal Cells Abstract: In this lab‚ you will find out how plant and animal cells are alike and how they are different. The onion elodea cells were both plants‚ so they had a cell wall. The cheek cells were animal cells‚ so they did not have a cell wall. Eukaryotic cells contain structures called organelles that carry out life processes. Eukaryotic cells can be classified by the types of organelles they contain
Premium Cell Organelle Eukaryote
and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
Premium Western blot Molecular biology Cell division
Methods for studying cells in the lab Centrifugation A centrifuge is a device for separating particles from a solution according to their size‚ shape‚ density‚ viscosity of the medium and rotor speed. Centrifugation can be used to separate cells from one culture or to isolate an organelle from the rest of the cell. The process relies on speed; the faster and longer the particle is centrifuged‚ the smaller the particles are that will be separated. Separation occurs by causing the larger particles
Premium Density Liquid Population density
shriveled remnant of the child’s. True False 5. In the blood hydrophillic hormones are often bound to transport proteins. True False True False 6. People with Type I diabetes mellitus have normal insulin levels. True False 7. Taste cells are not neurons. True False 8. Hypoglycemic hormones are secreted in response to excessively low blood glucose levels. True False 9. Suppose you were
Premium Nervous system Sensory system Sense
Lab design –Voltaic Cells Aim Of Experiment In this experiment‚ we are going to make a battery by making an electron current between the metals Zinc and Copper. We aim two study the voltaic cells and the transfer of electrons from the metal to the ion. The tendency for the electrons to flow from one chemical to another‚ such as from the zinc metal to the copper ion as shown here‚ is something that can be channeled and controlled. Channeling the flow of electrons is what we will take up in this
Premium Copper Potassium Metal