Aim: To verify the buffering capacity of a phosphate solution using a pH meter and construct a titration curve of an amino acid to determine the pka values of its ionisable groups to identitfy an unknown amino acid. Method: The ratio of [HPO42-] to [H2PO4-] required to produce buffer solutions at pH values 5.9‚ 6.9 and 7.9 were calculated. 0.1M of H2PO4- and 0.1M HPO42- were used to mix appropriate volumes to 25mL of each of the buffer solutions. The calibrated pH meter was used to measure
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ACTIVITY 1 Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. __The size and the concentration. ___ 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? __ The molecules were to large to go through. My results matched._ 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your
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* The lack of aldolase B can results in the accumulation of fructose 1 phosphate in the liver. This seems to be toxic and can cause death of liver cells. The short of aldolase B can cause the reduction of dihydroxyacetone phosphate and this will lead to decrease in phosphate level in the body. ( Monique L 2008) * The damage of liver cells and a decrease level of phosphate groups will lead to hypoglycemia and will damage
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----------------------------- 9. The result with 0.1% Glucose was a flesh-colored solution. 10. Ribose is one of the many vital carbohydrates necessary for our bodies to make ATP (adenosine triphosphate)‚ which is the energy source used by our cells. Phosphate Analysis 11. After heating‚ a cloudy white solution with white precipitate was the result. 12. Phosphorus forms a wide range of sulfides‚ where phosphorus can be P(V)‚ P(III) or other oxidation states. Most famous is the three-fold
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Oct 2012 LSM 1101 Biochemistry of Biomolecules Class 1a: Nucleic acids Our class on DNA is divided into 3 parts: (I) Genetics (II) DNA structure (III) Concepts and applications. I. Genetics: In the primordial period‚ simple molecules were formed from atoms and from these molecules‚ macromolecules were formed. These macromolecules formed life and all living organisms. The classical genetic and heredity observations in the 19th century started the search for the origin of life. The transforming
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TITLE AND AUTHOR Lab 7 Analysis of purified Concanavalin A via:Hemagglutination INTRODUCTION The purpose of this lab was to test the biological activity of ConA by performing a hemagglutination assay. If ConA is active then agglutination will occur due to ConA’s free receptors being able to bind to the glucose residues on the sheep’s red blood cells. If ConA is not active then no agglutination will occur. To test the hemagglutination reaction‚ two types of ConA solutions
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01) The Cell Structure and function Abstract In this lab the structures of various cells were viewed through a compound microscope. The bacteria in yogurt was viewed a long with a slice of potato‚ and a thin skin of red onion. The potato was dyed with iodine while the red onion was dyed with methane blue. The students had to assemble the first three slides. Two more slides were provided these are the yeast and protozoa cells. Those two are also viewed
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what I accomplish in the campus reflects my personality. I decided to study biochemistry because I want to make a change in this world‚ and also because I want to serve the humanity through harnessing the fields of biology and chemistry to ameliorate people’s health and to develop new technologies that will improve industrial processes. Possessing millions of dollars will support my post studies in chemistry and biochemistry branches‚ which will enhance my level of knowledge in these fields‚ thus having
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BioLab3 Cell Membranes Lab Report Student Name: I. Diffusion Define the following terms. Solvent Solute Solution. Diffusion Concentration gradient Dynamic equilibrium EXERCISE 1 – Factors influencing rate of diffusion Predict how molecule size and temperature will affect the outcome of this experiment. Record the data from the information in the lab. Potassium Permanganate R.T. Methylene Blue R.T. Time (min) Total Diameter
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Under Microscope Lab Report A. The purpose of the investigation is to determine the time an onion cell spends in each part of the cell cycle. B. The significance of this investigation is to help us understand and know how long cells are undergoing mitosis. My personal interest is to know the phases of mitosis in cells in order to someday help find cures for cancer. C. The cell cycle is a chronological set of events‚ culminating in cell growth and division into two daughter cells. Some of the reasons
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