number of unsaturated bonds e) must contain the same functional group 3. In living organisms‚ buffers do which of the following? a) prevent charged particles from entering cells b) neutralize the effect of enzymes c) maintain pH in the cells within a narrow range‚ allowing enzymes to function d) allow the pH of cells to change without affecting the action of the enzymes e) maintain cytoplasm in a fluid condition 4. When atoms from two different elements are brought together under conditions
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BIOCHEMISTRY REVSION PROTIEN Question (1): Define and distinguish primary structure‚ secondary and tertiary structures of proteins. Protein Structures: Primary structure Primary structure of protein is its unique sequence of amino acids forming its polypeptide chain. The primary structure of a protein is starting from the amino-terminal (N) end to the carboxyl-terminal (C). Secondary structure Most proteins have segments of their polypeptide chain repeatedly coiled of folded in patterns
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References: * Bradford‚ M.M. “A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Dye Binding.” Analytical Biochemistry. 1976‚ 72‚ 248–254. * Spector‚ T. (1978). “Refinement of the Coomassie Blue Method of Protein Quantitation.” Analytical Biochemistry. 1978‚ 86‚ 142–146.
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Result: Part A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired
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vincristine PEB: Cisplatin (platinum)‚ etoposide‚ bleomycin VAD: Vincristine‚ doxorubicin (adriamycin)‚ dexamethasone General Cancer is basically a disease of cells characterized by a shift in the control mechanisms that govern cell proliferation and differentiation. Cells that have undergone neoplastic transformation usually express cell surface antigens that may be of normal fetal type‚ may display other signs of apparent immaturity‚ and may exhibit
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PH 131 BIOCHM FNDTN THERAPEUTICS DR. JOSEPH 1. Select the predominant form of Amoxicillin (shown above) at pH 5.3. The pKa of the phenolic -OH is 9.6‚ the NH3+ is 7.4‚ and the COOH is 2.4. 2. At what pH would amoxicillin most readily cross a chloride channel? A. 1.8 B. 5.7 C. 8.0 D. 10.5 E. 3.6 3. Using the pK values shown in parentheses in the above diagram‚ select the pI of the tripeptide Arginiyltyrosinylcystyl from the following choices: A. 11.6 B. 5.0 C. 5.6
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Available at‚‚http://laposlton.wikispaces.com/file/view/Qualitative+Testing+for+Amino+Acids+%26+Proteins.pdf(Accessed on 2nd of November 2013) 2. ‚University of Arizona‚Sulphur containing Amino acids ‚2003‚Available at ‚http://www.biology.arizona.edu/biochemistry/problem_sets/aa/Sulfur.html (Accessed on 31st of October 2013) 3. Value @ Amrita‚Qualitative Analysis of Amino Acid‚2013‚Available at ‚http://amrita.vlab.co.in/?sub=3&brch=63&sim=1094&cnt=1 (Accessed on 30th October 2013) 4. Test for Amino acids
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Cell Transportation Lab Eggs are a great example of a cell. Underneath the hard shell is a thin membrane that is just like the cell membranes in your cells. There are microscopic pores in the membrane that allow substances to move in and out of the cell. Way Cool! Recall that the function of the cell membrane is to control the internal balance of the cell. It helps to maintain homeostasis. In this lab you will use vinegar‚ an acid‚ to dissolve the shell off of the egg to expose the membrane
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BCH 369L–Biochemistry Laboratory Fall 2016 Module C Orlando Martinez uteid: olm298 Introduction The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin
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Introduction Using microscopes allows humans to see things they’ve otherwise would have never seen before‚ like cells. A cell is the basic unit of life. All living things are made of cells. All cells come from preexisting cells through a process called cellular division. There are two types of cells‚ eukaryotes and prokaryotes. Prokaryotes are very simple and small. They are unicellular and have no membrane bound organelles. Their DNA is found directly in the cytoplasm since they have no nucleus
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