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    Microbiology Case Study

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    nothing out of the ordinary except dehydration from the diarrhea. Lab tests showed that he had 13‚000 white blood cells/microliter of blood‚ and a methylene blue stain of his feces showed abundant neutrophils. His stool specimen was plated on MacConkey agar and several colonies on the plate turned bright pink after 24 hours. Based on symptoms and the lab results‚ he was treated with trimethoprim-sulfamethoxadole and intravenous fluid therapy. He improved quickly and was released after 24 hours.

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    Today‚ certain antibiotics are used in fighting certain diseases caused by pathogenic bacteria such as Salmonella enterica and Escherichia coli. However‚ due to the improper usage of these antibiotics and the increasing antibiotic resistance of the bacteria‚ a lot of the medicines commonly used before to fight the same bacteria are now considered ineffective and because of it‚ scientists try to find ways such as discovering new antibiotics or recommending to the citizens some procedures and ways

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    Microbiology Lab Report

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    identify their bacteria. During the process of identification‚ the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB)‚ TSI (Triple Sugar Iron agar)‚ Phenol red sucrose‚ the SIM test‚ H2S by SIM‚ IMViC (indole‚ motility‚ voges-proskauer‚ and citrate)‚ Urease (urea broth)‚ PDase (Phenylalanine Deaminase)‚ Lysine Decarboxylase‚ and Ornithine Decarboxylase. Colonial morphology on EMB was used to

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    Doctors use this to determine which antibiotics are needed to treat infections. b) The test is used to see how the antimicrobials impregnate on the disks are then placed on a plate which then are incubated to grow more bacteria it also allows the agar to diffuse over. Then a concentration gradient is created‚ if there is a zone of inhibition the the disks will show the growth being

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    introduction of mung bean

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    of cellophane noodles‚ called mung bean sheets or green bean sheets‚ are also available. In Korea‚ a jelly called nokdumuk (hangul: 녹두묵; also called cheongpomuk; hangul: 청포묵) is made from mung bean starch; a similar jelly‚ colored yellow with the addition of gardenia coloring‚ is called hwangpomuk (hangul: 황포묵). In northern China‚ mung bean jelly is called liangfen (凉粉‚ meaning chilled bean jelly)‚ which

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    Microbes are everywhere. Objectives: The experiment performed in the Lab was isolation of microbes taken from us and the environment. We used Nutrient Agar which is a growth medium used to culture microorganisms or small plants and Sabourand Dextrose Agar plates used to cultivate moulds and yeasts. The objective of it was to demonstrate that microbes are everywhere. We expected to find a variety of bacteria‚ moulds and yeasts. We were introduced to aseptic techniques as they help ensure that

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    generating diversity in life and aiding in evolution. Materials and Methods The materials used in the lab were two agar petri plates‚ a permanent marker‚ labeling tape‚ sterile scalpel‚ compound microscope‚ toothpick‚ microscope slide‚ water‚ cover slip‚ and the ascospore Sordaria cultures‚ which included wildtype (dark)‚ tan‚ and grey (mutant) fungi. First‚ collect two agar plates to make the cross between the three different colored ascospores. The Sordaria should be crossed in the following

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    Petri dish- This is used to transfer the agar cubes as you should put the cubes in there as some of the chemicals can be dangerous Safety goggles- These are essential to keep your eyes protected from harmful chemicals White tile- To help to see the colour change Stop clock- To time how long it takes for the cube to change colour. Method 1-Stop the clock and quickly but carefully place one of the agar cubes into the specimen tube containing 3.7g/100cm3 of hydrochloric acid

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    Stem Cell

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    Mesenchymal cells isolation from Wharton’s jelly‚ in perspective to clinical applications Iro KOLIAKOS 1*‚ Nikos TSAGIAS 2 and Vassilis KARAGIANNIS 2 1 2 Stem Cells Bank‚ National Research Foundation‚ Athens‚ Greece C Gynecology Clinic‚ Hyppokrateion Hospital‚ Medical School‚ Aristotle University of Thessaloniki‚ Greece Received: 16 September 2010 Accepted after revision: 22 November 2010 Human umbilical cord tissue‚ termed as Wharton’s jelly‚ is known to be an important source of

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    Ubiquity of Bacteria

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    a colony count. Sterile cotton swabs were used to sample the classroom telephone‚ light switch‚ cell phone‚ and a student’s tongue. After carefully swabbing each site‚ we inoculated separate Petri dishes and a test tube of nutrient broth. A blood agar plate was exposed to bacteria by coughing on it. The plates were then incubated at 37ºC for 48 hours. The light switch and telephone were contaminated with both bacteria and mold which produced turbid tubes of broth. However‚ the sampling of the

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