Gene Mapping in Ascospore Sordaria Cultures by Recombination
Gene Mapping in Ascospore Sordaria Cultures by Recombination
Abstract
The laboratory experiment demonstrates the process of meiosis using the Sordaria Fimicola fungi. Meiosis is important because it is the process that generates diversity in genetics. A wildtype was crossed with two mutant types: tan and grey. In order to exhibit recombination, the sequence of ascospores needed to result in a 2:2:2:2 or 2:4:2 sequence. From the crossover event, the percentage of asci that had this pattern was used to calculate the distance from the gene to centromere in map units. The distance or location is a significant because it can affect the frequency of crossing over and thus recombination. Results suggest there was less recombination, meaning there were unusual arrangements such as 3:1:1:3.
Introduction
The purpose of the Sordaria lab is to demonstrate the outcome of meiosis and its importance using a haploid organism such as Sordaria (fungi). Using favorable conditions, the Sordaria was able undergo sexual reproduction allowing for the creation of a diploid organism. The crossing of a wildtype and mutant fungi will further exhibit whether or not recombination occurred. Recombination is important because it ensures that chromosomes exchange segments resulting with genetically unique gametes. This process is important for generating diversity in life and aiding in evolution.
Materials and Methods
The materials used in the lab were two agar petri plates, a permanent marker, labeling tape, sterile scalpel, compound microscope, toothpick, microscope slide, water, cover slip, and the ascospore Sordaria cultures, which included wildtype (dark), tan, and grey (mutant) fungi.
First, collect two agar plates to make the cross between the three different colored ascospores. The Sordaria should be crossed in the following way: wildtype x tan, wildtype x grey. On the bottom
Abstract
The laboratory experiment demonstrates the process of meiosis using the Sordaria Fimicola fungi. Meiosis is important because it is the process that generates diversity in genetics. A wildtype was crossed with two mutant types: tan and grey. In order to exhibit recombination, the sequence of ascospores needed to result in a 2:2:2:2 or 2:4:2 sequence. From the crossover event, the percentage of asci that had this pattern was used to calculate the distance from the gene to centromere in map units. The distance or location is a significant because it can affect the frequency of crossing over and thus recombination. Results suggest there was less recombination, meaning there were unusual arrangements such as 3:1:1:3.
Introduction
The purpose of the Sordaria lab is to demonstrate the outcome of meiosis and its importance using a haploid organism such as Sordaria (fungi). Using favorable conditions, the Sordaria was able undergo sexual reproduction allowing for the creation of a diploid organism. The crossing of a wildtype and mutant fungi will further exhibit whether or not recombination occurred. Recombination is important because it ensures that chromosomes exchange segments resulting with genetically unique gametes. This process is important for generating diversity in life and aiding in evolution.
Materials and Methods
The materials used in the lab were two agar petri plates, a permanent marker, labeling tape, sterile scalpel, compound microscope, toothpick, microscope slide, water, cover slip, and the ascospore Sordaria cultures, which included wildtype (dark), tan, and grey (mutant) fungi.
First, collect two agar plates to make the cross between the three different colored ascospores. The Sordaria should be crossed in the following way: wildtype x tan, wildtype x grey. On the bottom