Yearin Kwon 2nd Period 12. 01.2012 Research Paper E Coli The Escherichia Coli 0157:H7 or also know as E Coli‚ is a food born pathogen that can cause serious illness to humans at any age. The E Coli can also lead to death if left untreated. There are many symptoms with the E Coli. And one of the most obvious symptoms is having a bloody diarrhea (Not all forms of E Coli have the same symptoms). The symptoms can be easily confused with other disease since it is such a common symptom. The
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sold. Recall products is a returned product after a discovery for a safety issue. It can endanger a customer that has bought the product and will affect them with a deadly bacterium‚ E. Coli O121. Escherichia Coli is a bacteria found in the environment‚ foods and intestines of people and animals. The bacterium E. Coli O121 can cause bloody diarrhea and dehydration. When symptoms are noticed‚ seek for emergency medical care right away. There are other food diseases that can cause a poor sickness. The
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This experiment tested the growth of E.coli with inserted plasmid on an agar plate with Ampicillin. One colony of E.coli resistant to Ampicillin was grown during this experiment. The overall goal of the experiment was to successfully grow E.coli on the agar plate‚ which would show that the plasmid had been effectively inserted into the bacteria’s genes. This experiment helped students understand how plasmids were inserted into bacteria and used in real life situations. It also showed how the bacteria’s
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Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another‚ through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith‚ a British microbiologist. Griffith observed that the mutant form‚ non-virulent form‚ of the bacteria Streptococcus Pnumoniae could be transformed into the normal‚ virulent form‚ when injected into mice along with heat killed normal forms. He concluded that somehow
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Transformation Of Escherichia Coli With pGLO Plasmid April 24‚ 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait‚ in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid‚ which carries a gene for ampicillin
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The Escherichia Coli bacterium was discovered in 1885 by bacteriologist Theodor Escherich. He found the bacteria in the human colon and showed that some strains of Escherichia Coli were responsible for infant diarrhea and gastroenteritis. The Escherichia Coli was originally named Bacterium Coli. The name was changed to honor Theodor Escherich. E coli are normally harmless and are part of the normal flora found in the lower intestines of humans and healthy cattle. Some E. coli serotypes are known
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four different colonies of E. coli bacteria. Two of the colonies will be grown on plates with ampicillin and Luria Broth which is composition of ingredients used to promote growth of‚ in this case‚ the bacteria. One of these plates will contain plasmid‚ while the other will not. The other two plates will just contain Luria Broth. Once again‚ one will contain plasmid and one will not. The research question for this experiment is: What is the difference in the transformation efficiencies between pFLO
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METHODS In this experiment‚ plasmid lux and a control plasmid (pUC18) will be introduced into E. coli by transformation. There are four basic steps to the procedure; Preparation of competent cells (These steps should be performed by the instructor.) 1. Place a vial of CaCl2 solution and the tube of E. coli in the ice bath. 2. Using a sterile pipet‚ transfer 590µL CaCl2 solution to the tube containing 50µL of the bacteria. 3. Tap the vial with the tip of your index finger to mix the solution. 4. Incubate
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E. coli O157H7 What is the morphology and gram reaction of this pathogen (2) E. coli‚ including E. coli O157H7 is a gram-negative bacillus. What do (i) O157 and (ii) H7 attached to the name of this bacterium represent (2). The O157 is the O HYPERLINK http//www.emedicinehealth.com/script/main/art.asparticlekey5469 serotype antigen that identifies the E. coli strain‚ and the H7 represents the antigen type on the HYPERLINK http//www.emedicinehealth.com/script/main/art.asparticlekey2416 bacteriums
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In the experiment involving varying pH levels‚ E. faecalis‚ S. epidermidis‚ L. lactis‚ and L. casei were subjected to different pH levels and then were allowed to incubate in order to determine the minimum‚ maximum‚ and optimum pH levels for growth of specific bacterial species. It was found that lower pH levels between 2 and 4 inhibited or promoted little to no growth for E. faecalis‚ S. epidermidis‚ and L. casei. It is evident that these bacteria were resistant to alkaline environments‚ however
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