How will a microbiologist use this staining method to begin the identification of bacteria? Microbiologists use this staining method to begin the identification of bacteria because it will allow them to tell the four different categories based upon color reaction and shape. It is the first step that will help in identifying bacteria
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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D4 H and E staining procedures Haematoxylin and eosin staining is a technique used by pathologists to add definition to nuclei and other parts of a cell in tissue samples under a microscope so that they can see it better when analysing the cells form and abnormalities. The H&E technique is the most commonly used in histology and the diagnoses of malignancies is based largely on the results of this procedure. The dyes will specifically stain elements of a cell or tissue because the dyes have a high
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The problem that was required to be investigated in this laboratory experiment was to determine which type of cleaning product would be able to prevent the most bacterial growth when placed in a neutral environment. In order to answer this problem‚ 8 different disinfectants were tested‚ including sterile water‚ and their after two days their zones of inhibition were measured and recorded. A hypothesis that was formulated before experimenting was‚ “If the type of disinfectant is Mouthwash‚ then it
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NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for 30 seconds
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bacteria do not maintain the crystal violet dye used in the gram staining method because of their cell walls that are thin .most of the gram negative bacteria are usually harmful to the host. (Gram negative rod shape salmonella entrica) There are several tests and experiments that classified the genus of the unknown species‚ which known as gram staining method. Carbohydrate fermentation: 1) Phenol red lactose broth
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram positive but your gram stain results indicated that it was gram negative‚ how would you proceed and can you think of any reasons why this may
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Del Mundo‚ Layah Basella rubra as a biological stain Experimental The alugbati extract turned out to be favorable stain for plant nuclei and organelles especially for Hydrilla verticillata and as substitute for crystal violet or safranin in Gram staining. The extract stained the bacteria red-orange and the plant nuclei‚ and the cytoplasm red and light pink‚ respectively. The study is limited to the ability of Basella rubra (alugbati) to be used as a biological stain 2011 Gel-O-Fury- Saavedra‚ Ralph
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are acidic dyes since they do not cross the cell wall/membrane particularly easily. A background stain is a stain used to stain everything other than the bacteria. The Gram stain is one of the oldest‚ most cost-efficient‚ yet most under-utilized‚ staining method used to identify bacteria. It consists of a basic dye (the primary stain crystal violet)‚ a mordant (Gram ’s iodine)‚ decolorizer (acetone:alcohol) and a counter stain (safranin). A mordant is a compound which helps hold the primary stain
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