Theories of Staining Techniques Staining bacteria with different dyes via staining techniques‚ allows in distinguishing the microorganism from its backgrounds. Also‚ helps in studying different internal structures such as vacuoles‚ cell walls and spores in details (Seeley and others 1991). Some staining techniques such as Gram staining‚ endospore stain and capsule stain are some of the theories of stains used in bacteriology today. Also‚ these staining procedures help in determining properties
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Unknown #1 Vomit Serratia marcescens & Bacillus cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The
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Investigation of the probiotic properties of bacterial strains from two probiotic drinks and their survivability in artificial gastric juice ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria‚ but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating
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Experiment 2: Microscopy & Simple Staining September 30‚ 2013 General microbiology laboratory The invention of the microscope has opened up a whole new dimension in science. By using microscopes scientists were able to discover the existence of microorganisms‚ study the structure of cells‚ and see the smallest parts of plants‚ animals‚ and fungi. Today‚ the microscope is still a commonly used tool to diagnose illness in hospitals and clinics all over the world (1)
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1.What is the purpose of staining samples?A) To increase the contrast and visibility of the specimen B) To kill the specimen C) To see the specimen without the aid of a microscope D) To make the specimen appear larger in the microscope2.Which of the following are bacterial SHAPES? (Select all that are correct. Be sure to select the appropriate scientific name. You lose points for choosing incorrectly or for not choosing a correct choice.)A) Vibrio B) Strepto C) Spirillum D) Staphylo E) Cocci
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ASEPTIC TECHNIQUES AND CELL COUNTING INTRODUCTION:- Microbes are single celled organisms and are so small that we cannot see with our naked eyes‚ microbes can only seen under microscopes .Microbes are the one of the oldest living form on earth‚ fossils of microbes were found which are said to be 3.5 billion years old . Microbe world: what is a microbe [online].[Accessed 9 April 2013].Available from: world wide web:‚microbes are found everywhere in rocks‚air‚water some of the microbes are even
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Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
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Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining‚ Capsule Staining‚ Endospore Staining‚ and Acid Fast staining. One of the most significant method of staining is the Gram Staining‚ as it is highly dependent
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1 1. Explain the principle of positive staining. (3 marks) Positive staining is done by staining the cell walls of Gram-positive bacteria with crystal violet. The cell wall of Gram-positive bacteria is made up of a thick layer of peptidoglycan which undergoes dehydration during decolourisation causing the pores to shrink. This shrinkage of pores traps CV-I complex and stain the cell purple. 2. Using a flow chart‚ outline the process of gram staining. (5 marks) Flood the slide with crystal
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morphologies were then observed and recorded‚ unknown A and unknown B‚ a Gram stain was then performed for each unknown. After determining the Gram reaction‚ alternate staining procedures were performed as needed. Unknown A required further endospore staining‚ whereas unknown B required no further staining procedures. Once all staining methods were complete‚ specific biochemical tests were performed. The biochemical tests were chosen from the unknown identification tables and dichotomous keys that
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