Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab‚ the Green Fluorescent Protein‚ which is typically found in the bioluminescent jellyfish Aequorea
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Catalog Number 166-0003EDU Week 7: pGLO Transformation Introduction to Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides the instructions for making (codes for) a protein. This protein gives an organism a particular trait. Genetic transformation literally means change caused by genes‚ and involves the insertion of a gene into an organism in order to change the organism’s trait. Genetic transformation
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Introduction In this week’s laboratory period students had the opportunity to perform a common procedure preformed by many if not all microbiologists known as genetic transformation. Genetic transformation is the ability to move DNA into an organism and thereby altering its genotypic and genetic makeup (2). Genetic transformation has shown to have a wide variety of uses in many scientific studies. In agriculture‚ gene coding for traits such as frost‚ pest‚ or spoilage resistance have been genetically
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Josh P. AP Biology 12-10-13 Lab Writeup The Effect of Transformation of pGLO in Bacteria Background/Objectives/Hypothesis: Genetic transformation is a process that primarily is inserting new DNA into an organism to change that organism’s trait. This process has many useful benefits when used correctly in different organisms. In this lab‚ bacteria was transformed by inserting DNA for Green Fluorescent Proteins. The DNA for these proteins were taken from bioluminescent jellyfish Aequorea
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Miguel Felix pGLO Transformation Mr. Betz AP Biology 14 December 2012 Abstract The purpose of this experiment is to determine the effects of the addition of a plasmid to a bacterial cell. The bacteria E. Coli was separated into two groups: one where the pGLO plasmid was added to the bacteria‚ which contains the genes of fluorescence and resistance to antibiotics‚ and the other lacking the plasmid. The two groups then placed in agar plates simulating different environments: the bacteria lacking
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The pGLO plasmid‚ which has the bla and GFP gene‚ was developed so it can operate like the arabinose operon and be able to transcribe genes in the presence of arabinose sugar. In this plasmid‚ the cluster of genes is regulated by the spontaneous on/off element by a single promoter‚ which is dependent on the DNA binding protein araC. This protein is at the binding site for RNA polymerase at the beginning of the operon‚ so when arabinose is present (like in one of the experimental plates)‚ it is taken
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CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
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Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
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Chem 105 Guide to the Formal Laboratory Report The purpose of a formal report is to communicate effectively to another person the goal‚ procedure‚ data analysis method‚ and results of your laboratory work. The report is divided into several well-defined sections. Each section must be present in a complete report. To earn an outcome point for the laboratory report‚ a student must submit a formal lab report that earns a score of at least 90/100. Each error (factual‚ grammatical‚ typographical
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Criteria 6 8 10 Title Your lab report has a Title that is directly related to the lab experiment/ exercise. Your group or class must be able to come up with your own title You must make your own title based on scientific theory Aim You have clearly stated your aim Your aim is relevant and informative You must make your own aim based on scientific theory Background background information relevant to the lab experiment/ exercise You must be able to link your aim to the background The
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