Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another‚ through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith‚ a British microbiologist. Griffith observed that the mutant form‚ non-virulent form‚ of the bacteria Streptococcus Pnumoniae could be transformed into the normal‚ virulent form‚ when injected into mice along with heat killed normal forms. He concluded that somehow
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In this lab‚ we performed a genetic transformation through the process of gene transfer. Gene transfer involves the insertion of a gene into an organism. The gene to be inserted is usually contained in a plasmid‚ which is relatively small‚ circular non-chromosomal DNA molecule typically found in bacteria. Once the plasmid containing the gene is inserted into the organism‚ it is absorbed into the organism’s own genetic code. After this occurs‚ the newly introduced gene begins coding for proteins‚
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Genetic transformation happens when an organism is altered by the introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia
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Malak Zomrawi 4/9/15 Bacterial Transformation I. Abstract In the lab‚ the purpose is to see if we could move genes using plasmid. As well as getting better understand of transformation methods using shock wave. To see the effects five trays are being used containing LB nutrient broth. The results showed that the LB‚ ampicillin‚ and arabinose with a positive pGLO had the most amount of growth compared to the other four trays. Although when there is arabinose there is no fluorescence‚ fluorescence
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Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab‚ the Green Fluorescent Protein‚ which is typically found in the bioluminescent jellyfish Aequorea
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166-0003EDU Week 7: pGLO Transformation Introduction to Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides the instructions for making (codes for) a protein. This protein gives an organism a particular trait. Genetic transformation literally means change caused by genes‚ and involves the insertion of a gene into an organism in order to change the organism’s trait. Genetic transformation is used in many areas
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Introduction In this week’s laboratory period students had the opportunity to perform a common procedure preformed by many if not all microbiologists known as genetic transformation. Genetic transformation is the ability to move DNA into an organism and thereby altering its genotypic and genetic makeup (2). Genetic transformation has shown to have a wide variety of uses in many scientific studies. In agriculture‚ gene coding for traits such as frost‚ pest‚ or spoilage resistance have been genetically
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Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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Miguel Felix pGLO Transformation Mr. Betz AP Biology 14 December 2012 Abstract The purpose of this experiment is to determine the effects of the addition of a plasmid to a bacterial cell. The bacteria E. Coli was separated into two groups: one where the pGLO plasmid was added to the bacteria‚ which contains the genes of fluorescence and resistance to antibiotics‚ and the other lacking the plasmid. The two groups then placed in agar plates simulating different environments: the bacteria lacking
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