to certain fungicides‚ for this experiment‚ a class of fungicides‚ the benzimidazoles (the MBCs) is used. 4.To determine whether the fungus has any polygalacturonase activity and whether this enzyme is affected by boiling. 5.To look at re-inoculation of the fungus‚
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I also sterilized the top of the test tube just for contamination purposes. Next I dipped my inoculation loop into the test tube to gather a sample of Staphylococcus aureus. Once I had my sample‚ I then began using the strike method to inoculate my TSA plate. I made sure to flame sterilize my loop between each strikes. I continued on using this same
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from genes UP-REGULATED by P. palmivora & “switch on” in roots & stem (primary targeted tissues) HO MW Cp9 Cp29 Cp35 Short life cycle Cp 29 - Beta-1‚3-glucanase Cp47 (actin) EtBr Easy to transform 24 hour post inoculation of P. palmivora MATERIALS & METHOD •Identify root and stem expressed genes from papaya. •Clone the 5’-upstream 2.0 kilobase regions containing putative promoters. 35 S Small genome (135Mbp) Cp9 •Assess promoter activity in Arabidopsis
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(-pGLO). 250ul of transformation solution which we used (CaCl2) was transfer to each tubes and placed those tubes on ice. HB101 bacteria single colony was picked by using sterile inoculation loop and immersed into (+pGLO) tube and later immersed into (-pGLO) using same technique. Both time we used different sterile inoculation loop. The tubes were placed back into the ice after mixing well the colony each time. The pGLO plasmid DNA was added by the instructor into (+pGLO) not into (-PGLO) tube and
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persistence of an inoculant strain on rice roots under greenhouse conditions. Growth responses to inoculation exhibited bacterial strain–rice variety specificity that were either stimulatory or inhibitory. Growth responses included changes in rates of seedling emergence‚ radical elongation‚ height and dry matter‚ plumule length‚ cumulative leaf and root areas‚ and grain and straw yields. Most notable were the inoculation responses toRhizobium leguminosarum bv. trifolii E11 and Rhizobium sp. IRBG74‚ which stimulated
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Test tube holder‚ 1 Test-tube-rack-6x21-mm‚ 1 Pipet Graduated Small (5 mL)‚ 1 Baker’s Yeast Packet – Saccharomyces cerevisiae‚ 1 Agar‚ MRS - 18 mL in Glass Tube‚ 4 Agar‚ Nutrient - 18 mL in Glass Tube‚ 1 Broth‚ Nutrient - 5 mL in Glass Tube‚ 2 Inoculation Loop‚ Plastic‚ 1 Mask with Earloops (11) in Bag 5" x 8" of Individual Colonies Methodology: Exercise 1: Isolation Using the Pour Plate Method Part I: Preparation of Solid Media 1. Disinfect the work area. 2. Melt the agar tubes. Refer
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bacteria. This will prove or disapprove on how to examine bacteria‚ and how to determine if a substance is contaminated. Materials: Yogurt Test Tube 95% Alcohol Dropper Permanent Marker Bunsen Burner Microscope Glass Slide Pipette Inoculation Loop Test Tube Safranin
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identify bacteria. These are referred to as the “five I’s” in your text. 2. Define / describe each of the following as they apply to microbiology: a. Culture f. pure culture b. Inoculum g. contaminated culture c. Inoculation h. mixed culture d. Colony 3. Microbiologists employee a number of approached to acquiring a pure culture from a from sample containing a number of different types of bacteria. Briefly describe three different procedures commonly
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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diseases -prevented but did not cure -promoted inoculation work‚ British were wrong b/c they denied Hunter/Gatherer Society Who: Natives and Europeans What: society where all food is obtained from wild When: 14th and 15th century Where: New World Significance: Natives were not technological advances as Europeans which gave them a disadvantage when it came to gathering food‚ hunting animals‚ and crops Inoculation Who: Nuns What: arm and nose inoculation When: Prior to 1796 Where: Asia‚ Africa
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