Preview

Bio Lab Report: Electro And Gram-Negative Contamination In

Good Essays
Open Document
Open Document
572 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Bio Lab Report: Electro And Gram-Negative Contamination In
Bio Lab Report

Title: Gram-Negative and Gram-Positive contamination in yogurt.
Introduction: There is bacteria that is beneficial and harmful in the human. Yogurt was choosed to be the best example that contains this bacteria. What happens to be the difference between these two types of bacteria, and how are they harmful? The bacteria is considered “Gram-Negative,” and the substance is considered contaminated when there is more negative bacteria than positive bacteria. This will prove or disapprove on how to examine bacteria, and how to determine if a substance is contaminated.

Materials:
 Yogurt
 Test Tube
 95% Alcohol
 Dropper
 Permanent Marker
 Bunsen Burner
 Microscope Glass Slide
 Pipette
 Inoculation Loop
 Test Tube
 Safranin

You May Also Find These Documents Helpful

  • Good Essays

    Botana Curus Lab

    • 569 Words
    • 3 Pages

    Next, we completed tests 1 and 2 wich was basically to oibserve the structural characteristics of the plants and seeds through a microscope . Then we came up with an hypothesis for wich species would be most closely related to the botana curus. Thirdly, we looked at the slides and comapared the arrangements of the bundles. Next we completed test four wich had to do with the chromatography paper and sepperating the different colored pigmentsfor each plant. Then, we did test five wich tested the plants for a fizz to check if they have enzyme M. Test six was the simulated gel electrophoresis that would compare the DNA. Lastly, we changed the sequence of DNA code to make amino acid to understand our…

    • 569 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Negative Unknown Lab Report

    • 2965 Words
    • 12 Pages

    The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli), Enterobacter aerogenes (E.aerogenes), Klebsiella pneumoniae (K.pneumoniae), Proteus mirabilis(P.miranilis), Pseudomonas aeruginosa(P.aeruginosa), and Salmonella typhimurium (S.typhimurium). The biochemical tests utilized were; Triple Sugar Iron Agar (TSIA) test, Sulfur Indole Motility (SIM) test, Methyl red test and Voges-Proskauer (MR-VP) test, Citrate test, Urea test,…

    • 2965 Words
    • 12 Pages
    Better Essays
  • Good Essays

    This experiment is to observe the significance of food safety. In addition, it will illustrate foodborne sicknesses and how food can be compromised by bacteria. This lab will culture washed and unwashed lettuce, as well as fresh unopened milk, and milk that has been opened for 7 days. The prediction for this lab, is that the unwashed lettuce and the opened milk will have the most bacteria growth. All results are meticulous within the expectations of the lab report.…

    • 630 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Allozyme analysis is a technique which is used in study of genetics because it reveals the genetic variation that exists within a wide range of organisms (Gómez, 1998). Allozymes are different forms of an enzyme expressed by alternative alleles on the same gene locus (Micales & Bonde, 1995). Analysis of these allozymes can be done by protein electrophoresis. Protein electrophoresis involves the movement of proteins within an electric field with mobility being dependent on factors such as the size and shape (secondary and tertiary structure), as well as the charge of the protein (due to primary structure). Other factors that can affect the mobility are electric-field strength, matrix pH, and ionic buffer strength of the electric field. Because there are so many factors involved in analyzing proteins during electrophoresis, it is unusual for two different proteins to have the same relative mobility (Gómez, 1998).…

    • 1385 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Through the process of gel electrophoresis, DNA fragments are able to be separated. Gel electrophoresis is a method of separating and analysis DNA molecule fragments based on their size and charge. On end of the gel is given a positively charged end and one end is negatively charged. When an electric current is passed through the gel charged molecules move through it. Larger molecules move slower, moving a shorter distance, while smaller molecules move faster and traveler further. These DNA molecules are separated by size in the gel and dye is used to stain the fragments and make them more visible.…

    • 231 Words
    • 1 Page
    Good Essays
  • Good Essays

    E. Coli Lab Report

    • 811 Words
    • 4 Pages

    The purpose of this lab is to successfully infiltrate E. coli bacterial cells with a pARA-R plasmid that is antibiotic resistant and has the rfp gene, or red fluorescent protein. This can be verified if the E. coli obtains the characteristics of the plasmid when it enters. To start, three Petri plates containing agar are needed. On each plate there is a control group and a treatment group; the treatment group being the one with the plasmid. Before the plasmid is put with the E. coli, first the bacteria are “stressed out” by warming them up in a hot water bath and cooling them down very rapidly in ice. The first plate consists of Luria Broth (LB), the second plate consists of LB and the antibiotic ampicillin (amp), and the last one contains LB, amp, and the sugar arabinose (ara). The bacterial cells are subjected to a heat shock and then are placed onto the three plates. The plasmid is spread on to only half of the first two plates, on the sides of the treatment group. Half of the E. coli get the plasmids and the other half do not (that side being the control group). On the third plate the plasmids are spread on the whole plate. The bacteria are left in an…

    • 811 Words
    • 4 Pages
    Good Essays
  • Better Essays

    MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us, we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms.…

    • 2623 Words
    • 11 Pages
    Better Essays
  • Good Essays

    MicroOrganism

    • 777 Words
    • 4 Pages

    9.) Be sure to note the morphology of the bacteria and whether it is gram-positive or negative.…

    • 777 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    The laboratory exam will be given on Tuesday December 4th (sections 0375 and 3252) or…

    • 371 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Micro Lab Project

    • 747 Words
    • 4 Pages

    It is very important to know not only what types of bacteria are in a sample but also how many of them are present. After a 2010 outbreak, founder Jimmy John Liautaud switched the sandwich chain to clover sprouts after Salmonella illnesses were associated with alfalfa sprouts. According to the Centers for Disease Control and the Food and Drug Administration, unwashed or lightly rinsed vegetables can harbor pathogenic bacteria and have been implicated in numerous food borne infections. Food manufacturers are required by the FDA to monitor the number and type of bacteria in their products. Very few of the things we eat or drink are bacteria free. They merely have greatly reduced numbers of “harmless” bacteria. It is often necessary to determine how many live bacteria are actually in a sample, especially when measuring growth rates or determining disinfectant effectiveness. This involves MacConkey agar which is a selective and differential…

    • 747 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Unknown Lab Report

    • 2145 Words
    • 9 Pages

    The tests performed on the unknown bacteria cultures were all used to determine the identity of the bacteria. Each of the tests performed provided some key information about the bacteria in question and how it functions. Not all of the tests were performed on every culture, however, as some of the tests were used only for gram (+) or gram (–) bacteria, while others were even more specific and used only for cocci bacteria. The tests performed and what constitutes a positive and negative test are as follows.…

    • 2145 Words
    • 9 Pages
    Better Essays
  • Good Essays

    One possible source of contamination may be the produce that is grown and distributed locally at a farmer’s market. A test sample of some of the produce revealed evidence of bacterial contamination. The bacteria sample was tested in a microbiology lab and showed the results that follow. The laboratory also performed a Gram stain of the isolated bacteria and ran a number of biochemical tests to aid identification. The biochemical tests were assayed using the Analytical Profile Index (API) 20E system for identification of Enterobacteriaceae and other gram-negative…

    • 891 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Dna Electrophoresis Lab

    • 1534 Words
    • 7 Pages

    To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also, to extract DNA from wheat germ.…

    • 1534 Words
    • 7 Pages
    Good Essays
  • Good Essays

    Identify Unknown Microbes

    • 550 Words
    • 3 Pages

    Next, an oxidase test was performed to eliminate one or more of the bacterium choices. P. aeruginosa was eliminated as the identity of the bacterium when the oxidase test results were negative. An EMB plate of the bacteria was examined to identify whether the bacterium was S. typhi or E. coli. The growth on the EMB plate was shiny and metallic green, meaning that it was positive for lactose fermentation. S. typhi exhibits intermediate lactose fermentation with pink or purple growth on an EMB plate, so it was eliminated as the possible identity of Bacteria A. To confirm the identity of Bacteria A as E. coli, an Enterotube test inoculated with Bacteria A was observed. The Biocode confirmed the identity of Bacteria A as E.…

    • 550 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria, but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating at different times. Actimel and Yakult were both able to survive the gastric juice. Actimel produced more colonies than Yakult but they both lost the same percentage of viability. The longer the time incubated the more the loss of viability.…

    • 2641 Words
    • 11 Pages
    Powerful Essays