Abstract The following experiment details the effect of different concentrations of catalase on the production of oxygen and water through the breakdown of Hydrogen Peroxide. In this experiment paper disc where coated in varying concentrations of catalase‚ 0‚ 25‚ 50 75 and 100%. The time taken for the disc to float between two markers on the side of a glass was then recorded. This experiment demonstrates that the higher the concentration of enzyme used the greater the production of oxygen on the
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measure the rate of respiration in yeast and find out what factors affect the rate of respiration. I am going to change the concentration of the glucose solution and I’m going to measure the volume of gas produced during respiration in cm³. Yeast contains enzymes. Enzymes speed up a chemical reaction – they’re biological catalysts. Yeast can respire both aerobically and anaerobic; the anaerobic respiration of yeast is known as fermentation. The equation for this type of respiration in yeast is:
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namely‚ alcohol fermentation and lactic acid fermentation. In alcohol fermentation‚ pyruvate (product of glucose in glycolysis) is converted to 2 molecules of ethanol (C2H5OH) and 2 molecules of carbon dioxide (CO2) while in lactic acid fermentation‚ pyruvate is reduced directly into lactic acid (Campbell and Reece‚ 2008). A good example of organism which produces ethyl alcohol and carbon dioxide through the process of alcohol fermentation is yeast (Madur‚ 2009). As a unicellular fungus‚ yeast is also
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by the concentration of starch‚ or whether the results I got were due to the amounts of solution used. This would make both my results and conclusion invalid and unreliable. I also made sure that the temperature of the water bath was at the correct temperature and I kept on checking the temperature of both my amylase‚ and starch using a thermometer. I made sure both solutions were at the same temperature at all times throughout my experiments‚ so that temperature would not have an effect on my results
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of Glucose in Yeast Cells Glucose is absorbed across the cell surface membrane (plasma membrane) of most cells. A convenient way to investigate this is to use a solution of glucose and a suspension of yeast cells. The amount of glucose taken up from the glucose solution by yeast cells in a fixed length of time can be measured. At the end of the fixed length of time‚ further uptake of glucose is prevented by transferring the yeast suspension to a boiling water bath to kill the yeast cells. If the
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THE EFFECT OF INHIBITORS AND MANIPULATION IN ENERGY PRODUCTION OF YEAST CELLS FOR GLYCOLYSIS AND FERMENTATION INTRODUCTION The aim of this experiment was to study the process of alcoholic fermentation in Saccharomyces cerevisiae (yeast cells) and measure the rate of Co2 production during anaerobic breakdown of the respiratory substrate‚ sucrose‚ in the yeast cells. The effects of an inhibitor on respiratory enzyme were also looked at. It is hypothesized that the five different reaction mixtures
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does the concentration of sugar have on the rate of respiration of yeast? Apparatus: 1. Stopwatch with a resolution of 0.001 seconds 2. 4 test tubes 3. 200 ml of yeast in a beaker 4. 200 ml of glucose solution 5. 500 ml of limewater 6. 2 delivery tubes 7. 1 test tube rack 8. 1 water bath set to 37°C 9. 2 pipettes 10. 3 measuring cylinders with a resolution of 1ml‚ ranging from 0-30ml. 11. 200 ml of water Hypothesis: The higher the concentration of sugar:
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The goal of this project is to test whether alcohol and caffeine has an effect on heart rate‚ using the freshwater crustacean‚ Daphnia magna‚ as the experimental subject. For humans‚ alcohol works as a depressant‚ slowing down the heart rate of the consumer; caffeine works as a stimulant‚ increasing the heart rate of the consumer. The heart depends on an internal pacemaker system to keep it pumping consistently and at the right speed. Alcohol disturbs this pacemaker system and causes the heart
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210 Comparing Fermentation Rate of the S. cerevisiae Yeast in the presence of MgSO4‚ NaF and Sodium Pyruvate Hypothesis In the fermentation of rate of yeast‚ S. Cerevisiae‚ there will be a higher/ faster rate of ethanol production‚ However‚ using catalytic enzymes would make the rate more faster‚ and MgSo4 will have a higher rate of CO2 than that of NaF and Sodium pyruvate as it act as a more better catalytic enzyme than the others. Methods Preparation of Tubes A solution of yeast and glucose was
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Rate of Osmosis Investigation Lab Title: A simple heading referring to your investigation Abstract: Write this section last! It is a brief paragraph or 2 which outlines the purpose‚ the method‚ the pattern of results and the conclusion you reached. It is an overall snapshot of the whole investigation. Introduction: Purpose: Biological Background: All living organisms are made of cells which are surrounded by a membrane. The cell membrane has many functions but the
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