Section 1 Purpose: The purpose of this lab was to separate plant pigments using chromatography‚ calculate Rf values using the collected data‚ and study photosynthesis with isolated chloroplasts. Light energy Light energy Background Information (Activity A): In photosynthesis‚ plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. It is an endergonic and anaerobic reaction. Critical to the process is chlorophyll‚ the primary photosynthetic pigment
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LABORATORY REPORT FOR BIO411 Experiment 5 : Cellular Respiration Experiment 6 : Photosynthesis Title: Cellular Respiration Objective: To observe and determine cellular respiration in yeast/onion cells. * Measure respiration rate using different substrates. * Measure respiration rate at different temperature. Introduction: In this laboratory experiment‚ we are given 3 task. The first one is respiration in yeast. Second is respiratory indicator and the third one is observing
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using 1-methylimidazole as the starting material by using the alkylation and metathesis synthesis methods. H1-NMR and Ion Chromatography (IC) analysis will be used to verify the characteristic and presence of impurities to determine the purity of
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was measured Predictions: Chlorophyll a absorbs best at a wavelength of 400‚ Chlorophyll b at 450‚ carotene at 500‚ and xanthophyll at 450. Materials & Methods: Experiment: First all the materials were gathered. Pigments were separated by chromatography paper. Pigments were loaded in a line on the paper. The paper was folded in a cylinder‚ stapled‚ placed in a cylinder with solvent‚ and sealed with wax paper until the pigments separated. After the pigments separated the paper was taken out to
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I. Experiment 3: SEPARATION TECHNIQUES II. Objectives: * To learn different methods and techniques of separating components of mixtures * To appreciate the importance of such separation techniques * To apply different methods and techniques in separating components of mixtures III. Results and Observations Separation of components with different solubilities * AMMONIUM CHLORIDE‚ SODIUM CHLORIDE‚ SAND the wt. of mixture with evaporating dish after 1st heating is?? g. And after
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The poly-A tail is in the 3’UTR. The stop codon is always upstream of the terminator More than one of a-d are false 2. Immunoprecipitation is based on a similar principle as: a) b) c) d) e) SDS-PAGE Ion exchange chromatography Size exclusion chromatography Affinity chromatography PCR 3. How is a Western blot different from a Southern Blot‚ as we covered in class? a) b) c) d) e) f) A Western blot uses a gel. A Western blot uses a membrane. A Western blot uses an antibody. A Western blot uses
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(e.g.‚ ClO2-‚ and ClO-) in biological fluids. These biomarkers can be used to indirectly measure chlorine dioxide exposure. The concentration of residual chlorite ion in vegetables and eggs treated with sodium chlorite was determined by UV-ion chromatography (Suzuki et al. 1997). Sodium chlorite was extracted with water and cleaned-up using C18 cartridge. The detection
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Today in lab I will separate mixtures of compounds into their constituent components using chromatography paper and an eluting solvent of salt water as well as rubbing alcohol. Materials and Methods: In this lab using the bag of FD&C dyes‚ bag of M&M’s‚ 2 packs of Kool-Aid ‚ and a pack of Clover Valley store brand food coloring I am going to place a drop of each dye at the bottom of the chromatography paper. For the experiment with the rubbing alcohol I also placed a drop of each dye‚ but used
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Name: Date: Course: Lesson: Unit: Chromatography Lab INTRODUCTION: In 2 to 3 complete sentences‚ explain the purpose of this lab – what are you going to learn in this lab? (2 points) HYPOTHESIS: Answer the following in complete sentences: (2 points) 1. List one extensive and one intensive property of marker ink. 2. Define mixture. MATERIALS: coffee filter Tape rubbing alcohol water 3 different color magic markers (not permanent) 3 identical tall‚ narrow
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Partition equilibrium of a solute between two immiscible solvents Two-dimensional thin-layer chromatography 2 2 3 6 Chapter 65 Quantitative methods of analysis Detection of end point in acid-alkali titration 8 Chapter 66 Instrumental analytical methods More about infrared spectroscopy More about mass spectrometry 12 15 Chapter 67 Contribution of analytical chemistry to our society Gas chromatography-mass spectrometry 32 PART XV ANALYTICAL CHEMISTRY Chapter 63 Detecting the presence
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