1A
Name: Date:
Course: Lesson: Unit:
Chromatography Lab
INTRODUCTION: In 2 to 3 complete sentences, explain the purpose of this lab – what are you going to learn in this lab? (2 points)
HYPOTHESIS: Answer the following in complete sentences: (2 points)
1. List one extensive and one intensive property of marker ink.
2. Define mixture.
MATERIALS:
coffee filter
Tape
rubbing alcohol water 3 different color magic markers (not permanent)
3 identical tall, narrow drinking glasses metric ruler
3 pencils
PROCEDURE:
1. Cut 3 strips of coffee filter. Be sure that the strips are much narrower and slightly taller than the drinking glasses.
2. In the center the strips, about 3 cm from one end, place a dot of the marker to be tested. The dots should be about 0.2 cm in diameter and dark enough to be clearly visible.
3. Place about 2 cm of water in each glass.
4. Tape the unmarked end of each strip to the center of a pencil so that the strip hangs down when the pencil is held horizontally.
5. Carefully insert the strips into the glasses, dotted end down. The dot must be above the water, and the sides of the coffee filters cannot touch the sides of the glasses.
6. Let the setup stand for 20 minutes. Follow procedure 8 as your setup stands.
7. Record your observations after 20 minutes in the table below. Measure Df (in cm) for the solvent. Measure the Ds (in cm) for each color that you see on the coffee filter (there may not be three). Calculate the Rf for each color using the equation: Rf = Ds/Df
8. Repeat steps 1–7, except place about 2 cm of rubbing alcohol in each glass instead of water.
9. Clean up your work area.
DATA: (8 points)
Brand of marker used:___________
Chromatography Data
Marker Color
Solvent: water
Df
Colors
Ds
Rf
Df
Colors
Ds
Rf
Df
Colors
Ds
Rf
Solvent: rubbing alcohol
Df
Colors
Ds
Rf
Df
Colors
Ds
Rf
Df
Colors
Ds
Rf
Include a
Course: Lesson: Unit:
Chromatography Lab
INTRODUCTION: In 2 to 3 complete sentences, explain the purpose of this lab – what are you going to learn in this lab? (2 points)
HYPOTHESIS: Answer the following in complete sentences: (2 points)
1. List one extensive and one intensive property of marker ink.
2. Define mixture.
MATERIALS:
coffee filter
Tape
rubbing alcohol water 3 different color magic markers (not permanent)
3 identical tall, narrow drinking glasses metric ruler
3 pencils
PROCEDURE:
1. Cut 3 strips of coffee filter. Be sure that the strips are much narrower and slightly taller than the drinking glasses.
2. In the center the strips, about 3 cm from one end, place a dot of the marker to be tested. The dots should be about 0.2 cm in diameter and dark enough to be clearly visible.
3. Place about 2 cm of water in each glass.
4. Tape the unmarked end of each strip to the center of a pencil so that the strip hangs down when the pencil is held horizontally.
5. Carefully insert the strips into the glasses, dotted end down. The dot must be above the water, and the sides of the coffee filters cannot touch the sides of the glasses.
6. Let the setup stand for 20 minutes. Follow procedure 8 as your setup stands.
7. Record your observations after 20 minutes in the table below. Measure Df (in cm) for the solvent. Measure the Ds (in cm) for each color that you see on the coffee filter (there may not be three). Calculate the Rf for each color using the equation: Rf = Ds/Df
8. Repeat steps 1–7, except place about 2 cm of rubbing alcohol in each glass instead of water.
9. Clean up your work area.
DATA: (8 points)
Brand of marker used:___________
Chromatography Data
Marker Color
Solvent: water
Df
Colors
Ds
Rf
Df
Colors
Ds
Rf
Df
Colors
Ds
Rf
Solvent: rubbing alcohol
Df
Colors
Ds
Rf
Df
Colors
Ds
Rf
Df
Colors
Ds
Rf
Include a