minute heating at 95oC to activate the polymerase and an additional 7 minute extension step at the end. Samples are kept at 4oC until taken out of the PCR machine. SYBR green was used and it binds to double-stranded DNA by intercalating between base pairs‚ and fluoresces only when bound to DNA. The detection occurs during the PCR cycle at the end of either the annealing or the extension step when the greatest amount of double-stranded DNA product is present. The standard curve was good beside the
Premium DNA Polymerase chain reaction DNA replication
cables types 6a cabling can do 10 GB over it‚ however 10 Gig switches are expensive. Cable structure UTP 4 pairs of copper cable Number of twists varies per inch N shielding Very easy to work with Most common cable for LAN Cat 3‚ Cat 5‚ Cat 5e‚ Cat 6‚ Cat 7 will be defined in standards later in the course Most susceptible to Electromagnetic Interference (EMI) STP Similar to UTP Pairs shielded Core shielded SCTP Coax Single conducting core FO Central glass core Cladding prevents light loss Single-Mode
Free Twisted pair Ethernet Computer network
Eric Scantlebury NT1310 Physical Networking Unit 3 Assignment 1: Specifications - Definitions 4/10/14 Application Describes cables‚ fire ratings and shielding Unshielded Twisted Pair (UTP) Types of Ethernet and phone cables twisted around each other to decrease interference from EMI. Has no shielding. Shielded Twisted Pair (STP) Same as the UTP cables except it is shielded in a metal sheath around it to stop external interference. Fiber-Optic Cable A cable that uses glass to transmit data using light
Premium Twisted pair Optical fiber Coaxial cable
NT1310 Physical Networking Unit 4 Exercise 1: Cabling – Definitions Horizontal Cable- The cabling between and including the telecommunications outlet and the horizontal cross-connect. Backbone Cable- A cable connection between telecommunications or wiring closets‚ floor distribution terminals‚ entrance facilities‚ and equipment rooms either within or between buildings. Patch Cords- Any flexible piece of cable that connects one network device to the main cable run or to a patch panel that in turn
Premium Cable Optical fiber Light
WANs 26.AC signal‚ DC signal 27.Electric cicuit connecting twonodes in a network 28.Full duplex transmission 29.Attenuation 30.Single-mode‚ Multi-mode cables 31.802.11g 32.CSMA/CA 33.Fiber optic cables 34.Shielded twisted pair cable vs. Unshielded twisted pair cable 35.Straight-through‚ Crossover cable 36.UTP‚ Coax‚ Single-mode (SM)‚Multi-mode (MM) cables – max. distance 37.Ethernet max distance per IEEE standard 38.Network troubleshooting using TCP/IP model Chapter 5 39.Ethernet
Premium Twisted pair Coaxial cable Cable
different ways. DNA is analyzed using a Southern Blot‚ which allows scientists to observe the base pair patterns. DNA fingerprinting can be used in a few different ways. First‚ to find out if the child belongs to a person DNA fingerprinting may be used. When a child is born‚ it inherits the VNTR’s from the father and the mother. VNTR’s are variable number tandem repeats‚ which is repeated sequences of base pairs. The VNTR patterns are used to determine the father or mother. Next‚ DNA fingerprinting allows
Premium DNA Molecular biology Genetics
CHAPTER ONE 1.0 SIWES INTRODUCTION The Students Industrial Work Experience Scheme (SIWES) is one of the Federal Government policies established under decree No 47 of 1971 of industrial training fund. It’s also a skill-acquisition programme designed for the tertiary institutions as a complementary programme to the theoretical education and laboratory and workshop practices engaged in by students in tertiary institutions which‚ by industrial standards‚ are inadequate or insufficient to serve the
Premium Computer network Ethernet Twisted pair
INDUSTRIAL TRAINING REPORT Submitted by:- Meenakshi Gupta 6040 1184751 Ece -5b LUDHIANA COLLEGE Of Engineering and Technology‚ Ludhiana Acknowledgement I would like to add a few heartfelt words for the people who were
Premium Network topology GSM Twisted pair
expressed a desire for your employees to be able to connect at speeds of 1Gbps. With those speed requirements‚ that really narrows your choices down to either a fiber optic wiring solution‚ or wiring your business with Category 5 or Category 6 twisted pair cable. Since you stated to me that cost is a main issue in what you will ultimately make your decision on‚ the fiber optic solution seems to me to be the least suitable solution for your company. The wiring is the most expensive of the choices‚ at
Premium Cable Ethernet Twisted pair
DRAFT COPY Hkkjr ljdkj GOVERNMENT OF INDIA jsy ea=ky; MINISTRY OF RAILWAYS (dk;Zky;hu iz;ksx gsrq) (For official use only) Different types of Networks in Indian Railways (FOIS/RAILNET) A MAINTENANCE HANDBOOK dSeVsd@‚l@izkSt@10&11@‚pch&usVodZ CAMTECH/S/PROJ/10-11/HB-NETWORK EkkpZ 2011 March 2011 Maharajpur‚ Gwalior DRAFT COPY Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Chapter 11 Appendix –A Appendix –B Introduction
Premium Ethernet Twisted pair