Exercise 1 Part a
Kayleigh Schmidt
Purpose 2: Exercise 1 Part A
Purpose:
The purpose of this lab is to explore inoculation, incubation, isolation, inspection, and identification. Inoculation is the placement of a small sample or cells or the material with cells on a culture media that helps these cells grow. Incubation involves the optimal growth temperature in a controlled setting and is when the cells start to become visible. There are two types of inspection, macroscopic and microscopic, and it helps to identify the microbes. Isolation is separating the microbe that is wanted onto a solid media and inspected further. Identification is the naming of the microbe. In this lab, inoculation, incubation and inspection are used. Inoculation is used because each TSA (tryptic soy agar) media was placed in a different place to collect cells as well as printed with a thumb print from each member of the group. Incubation is used because each sample needed a certain temperature for the cells to grow. Inspection was used to look at each plate and see how many colonies are found. One TSA plate was placed wherever the student wanted in the building and the other was for each group member to press their thumb print in. The SBA (sheep blood agar) was used for a mouth culture. The mouth culture was gathered by the student swabbing their cheek, tongue, and/or teeth with a sterile cotton swab. The TSA plates were to be incubated at 30 degrees Celsius for 24 hours and the SBA plate was to be incubated at 37 degrees Celsius for 20 minutes in the spot, but 24 hours in the
Purpose 2: Exercise 1 Part A
Purpose:
The purpose of this lab is to explore inoculation, incubation, isolation, inspection, and identification. Inoculation is the placement of a small sample or cells or the material with cells on a culture media that helps these cells grow. Incubation involves the optimal growth temperature in a controlled setting and is when the cells start to become visible. There are two types of inspection, macroscopic and microscopic, and it helps to identify the microbes. Isolation is separating the microbe that is wanted onto a solid media and inspected further. Identification is the naming of the microbe. In this lab, inoculation, incubation and inspection are used. Inoculation is used because each TSA (tryptic soy agar) media was placed in a different place to collect cells as well as printed with a thumb print from each member of the group. Incubation is used because each sample needed a certain temperature for the cells to grow. Inspection was used to look at each plate and see how many colonies are found. One TSA plate was placed wherever the student wanted in the building and the other was for each group member to press their thumb print in. The SBA (sheep blood agar) was used for a mouth culture. The mouth culture was gathered by the student swabbing their cheek, tongue, and/or teeth with a sterile cotton swab. The TSA plates were to be incubated at 30 degrees Celsius for 24 hours and the SBA plate was to be incubated at 37 degrees Celsius for 20 minutes in the spot, but 24 hours in the