MATERIAL AND METHODS Recombinant protein production The recombinant T-Rp3 was expressed in E. coli BL21 (DE3) as previously described (Favaro et al.‚ 2014) and purified by a single step His-based affinity chromatography in an ÄKTA purifier FPLC (GE Healthcare). Protein was then dialyzed against 50 mM MES buffer (pH 6‚0) overnight at room temperature‚ frozen in liquid nitrogen and stored at -80 ˚C after 0.22 μm pore membrane filtration. Evaluation of DNA-protein interaction by gel retardation
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The poem ‘The Love Song of J. Alfred Prufrock’ by T.S. Elliot is one that is full of literary devices that give the already deep piece of literature an entirely new depth when you look for them. The tone‚ plot‚ and overall theme of the story portrays a solemn and regretful lament of a man who has missed out on an integral part of his life‚ true love. J. Alfred Prufrock has coasted throughout his early life thinking he had plenty of time to do the things he hasn’t yet‚ found love and truly savored
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“The Love Song of Alfred J. Prufrock” is a poem written by T.S. Eliot‚ who introduces to the readers an overwhelming question‚ which pertains to whether the main character‚ Alfred Prufrock‚ should overcome his indecisiveness and fear or continue in his comfortable and dull existence. Prufrock is not happy with the decisions he has made in life‚ and that fact is very clear‚ as he tortures himself with internal conflict throughout the poem. In the beginning lines of the poem‚ he enthusiastically invites
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preferred math and science over English and did not enjoy English 102 very much. Despite my low expectations and preconceived notions of what English 103 would entail‚ I actually enjoyed myself this semester. Specifically‚ the poem The Love Song of J. Alfred Prufrock by T.S. Eliot changed my demeanor. I should start with the fact that I am a feminist and anything about equality‚ gender roles‚ or stereotypes intrigues me. This poem’s take on what true masculinity entails entranced me almost immediately
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In “The Love Song of J. Alfred Prufrock”‚ T.S. Eliot reveals a song of a split between shyness and passion. He gives his point of view about the frustration of living in a “fantasy world” being. While T.S Eliot took this poem as more to a man feeling insecure I took this poem as a love failure‚ as if a love that never happened and that would not ever happen because of lack of courage/bravery. The insecurities of the man took him to feel humiliated and rejected because of his age. This poem starts
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We just know needs of Small Business‚ let’s take a look at what the att providing for customers: At first‚ att is providing a fast and stable internet access‚ there are thousands of AT&T Wi-Fi Hot Spots in the country‚ customers can get access to the Basic Wi-Fi service We are providing: Wireless: customers Share Internet access‚ files‚ servers‚ printers‚ and other devices within their office. Customers can use our Most reliable 4G LTE network‚ the network has the Largest international coverage
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OPERATIVE REPORT Patient Name: Putul Barua Patient ID: 135799 Room: CCU4 Date of Surgery: 01/08/2013 Admitting Physician: Dr. Simon Williams‚ MD Pulmonology Surgeon: Dr. Simon Williams‚ MD Pulmonology Preoperative Diagnosis: Recent onset hemoptysis‚ history of tuberculosis. Postoperative Diagnosis: No tuberculosis lesions seen. Operative Procedure: Bronchoscopy. Specimens Removed: Blood clots. Indications: Patient requires bronchoscopy because of recent onset hemoptysis and a
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Mrs. J having all the sign and symptom with the history of having hypertension‚ chronic heart failure‚ sleep apnea‚ and smoke two pack of cigarettes a day‚ is not a thing to play with because she has expose herself to many things such as stroke‚ and brain death because the sleep apnea and the heart attach‚ will deprives the brain of oxygen since the cardiac muscle are not strong enough to be able to pump blood to her brain. Heart failure occurs when the cardiovascular system is unable to meet the
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ORGANISATIONAL STRUCTURE OF JOHNSON&JOHNSON JOHNSON & JOHNSON’s organizational structure is dictated by its corporate strategy. Johnson & Johnson has more than 250 companies located in 60 countries around the world. Johnson & Johnson Family of Companies is organized into several business segments comprised of franchises and therapeutic categories. Consumer HealthcareThe Consumer segment includes a broad range of products used in the baby care‚ skin care‚ oral care‚ wound care
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of J.curcas latex extract as potential antiparasite was tested against L3 H.contortus. In vitro anthelmintic activity of J.curcas was observed based on its ability to inhibit L3 H.contortus larval motility. Table## showed the larval inhibition (%) of J. curcas latex extract at various concentration against L3 H.contortus. To assess the anthelmintic performance of the plant‚ L3 H.contortus was exposed with the latex extract at concentration of 5mg/ml‚ 10mg/ml‚ 15mg/ml‚ 20mg/ml and 25mg/ml. The larvae
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