J Curcas Latex Extract Lab Report
The efficiency of J.curcas latex extract as potential antiparasite was tested against L3 H.contortus. In vitro anthelmintic activity of J.curcas was observed based on its ability to inhibit L3 H.contortus larval motility. Table## showed the larval inhibition (%) of J. curcas latex extract at various concentration against L3 H.contortus. To assess the anthelmintic performance of the plant, L3 H.contortus was exposed with the latex extract at concentration of 5mg/ml, 10mg/ml, 15mg/ml, 20mg/ml and 25mg/ml. The larvae were incubated for 48, 72 and 96 hours and the results were shown in Fig ##.
At 48hours of incubation, the larval motility started to decline at concentration 15mg/ml onwards. Where at concentration 15mg/ml, 20mg/ml and 25mg/ml, …show more content…
However, the positive control produced unexpected result where, albendazole at concentration 20mg/ml generated much lesser larval motility inhibition than the natural product used. At 96 hour incubation time, the result of positive control at 20mg/ml concentration was the same with the result generated by the latex extract of J.curcas at 5mg/ml concentration, which there is significant difference. Hence, the results provide further support for the hypothesis that J.curcas latex has a potent property to be antiparasite.
In general, through the observation made, the movements of the larvae were much slower and less active after incubated with J.curcas latex extract as compared to when the larvae were exposed to the albendazole. As the time increased, the larval motility was observed to be much slower. This might be due to the effect exerted by the latex extract. It was also observed, that the larvae were prolonging coiling themselves even after being exposed to the light and heat. According to Siamba,N.D. (2008), a physical change manifested as coiling is associated with the larvae response to gradual external stress. It was considered as a survival strategy aimed at conserving energy (lipid reserves) and protecting the parasite against molecular damage and ensures survival long enough beyond the span of the stressful period. Therefore, a possible explanation for this might be that the extract generated external stress to the larvae that may lead to paralysis or
At 48hours of incubation, the larval motility started to decline at concentration 15mg/ml onwards. Where at concentration 15mg/ml, 20mg/ml and 25mg/ml, …show more content…
However, the positive control produced unexpected result where, albendazole at concentration 20mg/ml generated much lesser larval motility inhibition than the natural product used. At 96 hour incubation time, the result of positive control at 20mg/ml concentration was the same with the result generated by the latex extract of J.curcas at 5mg/ml concentration, which there is significant difference. Hence, the results provide further support for the hypothesis that J.curcas latex has a potent property to be antiparasite.
In general, through the observation made, the movements of the larvae were much slower and less active after incubated with J.curcas latex extract as compared to when the larvae were exposed to the albendazole. As the time increased, the larval motility was observed to be much slower. This might be due to the effect exerted by the latex extract. It was also observed, that the larvae were prolonging coiling themselves even after being exposed to the light and heat. According to Siamba,N.D. (2008), a physical change manifested as coiling is associated with the larvae response to gradual external stress. It was considered as a survival strategy aimed at conserving energy (lipid reserves) and protecting the parasite against molecular damage and ensures survival long enough beyond the span of the stressful period. Therefore, a possible explanation for this might be that the extract generated external stress to the larvae that may lead to paralysis or