PSBPP103 Differential Gram’s staining EXPERIMENT NO. 1 AIM: THEORY: Page No: C1 Date: To Gram stain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. To study their properties and divide microorganisms into specific groups for diagnostic purposes
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employee a number of approached to acquiring a pure culture from a from sample containing a number of different types of bacteria. Briefly describe three different procedures commonly used to secure pure cultures from a mixed culture. The use of simple labeled diagrams may be quite helpful. 4. Microbiological media exists in a variety of forms and are classified on the basis of certain criteria. a. Describe the various physical states into which media are commonly classified
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endospores of living bacilli appear edged in black and are very bright and refractile. Endospores strongly resist application of simple stains or dyes and hence appear as nonstaining entities in Gram-stain preparations. However‚ once stained‚ endospores are quite resistant to decolorization. This is the basis of several spore stains such as the Schaeffer-Fulton staining method which also differentiates the spores from sporangia and vegetative cells. However this method is not suitable for taxanomic
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microbiology is the microscope. One of the first to use a microscope to observe microorganisms was Robert Hooke‚ the English biologist who observed algae and fungi in the 1660s. In the 1670s‚ “Anton van Leeuwenhoek‚ a Dutch merchant‚ constructed a number of simple microscopes and observed details of numerous forms of protozoa‚ fungi‚ and bacteria” (Introduction to Microscopes‚ n.d.). During the 1700s‚ microscopes were used to further explore on the microbial world‚ and by the late 1800s‚ the light microscope
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prokaryotes are and The two (2) genera of bacteria that are capable of producing spores are and b) c) d) An example of a Gram positive bacteria is example of a Gram negative bacteria. and is an e) Three (3) common differential staining methods used in microbiology are ‚ and "Ubiquitous" in microbiology means The minimum inhibitory concentration (MIC) means Of more than three thousand (3000) species of bacteria‚ only 10% cause disease. The three (3) diseases and their causative
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tuberculosis distinguishable from nearly all other bacteria. Acid-fast staining is‚ therefore‚ known as a differential stain. Mycobacterium and some Nocardia species are considered acid fast because‚ during the acid-fast procedure‚ they are able to retain the primary dye even when decolorized by a powerful solvent known as acid alcohol. Most other bacterial genera are easily decolorized by acid alcohol. Ziehl/Neelsen Acid-Fast Staining Procedure In later years‚ Ehrlich’s technique was improved upon
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image. Studyblue.com. STUDYBLUE INC.‚ 22 Apr. 2013. Web. 3 Nov. 2013. Reynolds‚ Jackie. Selective and differential media. Digital image. Www.microbelibrary.org). Kern Community College District‚ 12 Sept. 2011. Web. 3 Nov. 2013. Rose‚ Scott. B. cereus simple stain. Digital image. Napa Valley College. The Western Association of Schools and Colleges Designated Hispanic Serving Institution (HSI)‚ US Department of Education‚ n.d. Web. 5 Nov. 2013. Rose‚ Scott. Gram Stain B. cereus. Digital image. Napa Valley
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Theories of Staining Techniques Staining bacteria with different dyes via staining techniques‚ allows in distinguishing the microorganism from its backgrounds. Also‚ helps in studying different internal structures such as vacuoles‚ cell walls and spores in details (Seeley and others 1991). Some staining techniques such as Gram staining‚ endospore stain and capsule stain are some of the theories of stains used in bacteriology today. Also‚ these staining procedures help in determining properties
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Purpose/Question being addressed: The purpose of this experiment is to determine which kind of drinks stain your teeth the most. The drinks that will be tested are tea‚ coke‚ and coffee. B. Research Facts/ Hypothesis: Research shows that the darker the drink is that you drink the yellower your teeth will become. Teeth are naturally white or off-white in color but build- up from foods and drinks such as coffee and tea over
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1.What is the purpose of staining samples?A) To increase the contrast and visibility of the specimen B) To kill the specimen C) To see the specimen without the aid of a microscope D) To make the specimen appear larger in the microscope2.Which of the following are bacterial SHAPES? (Select all that are correct. Be sure to select the appropriate scientific name. You lose points for choosing incorrectly or for not choosing a correct choice.)A) Vibrio B) Strepto C) Spirillum D) Staphylo E) Cocci
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