Unknown #1 Vomit Serratia marcescens & Bacillus cereus Biol 251 Microbiology 5/14/2009 Introduction The purpose of this study is to differentiate and identify two unknown organisms provided by the instructor in a nutrient broth. It is only known that the two organisms are from vomit; one is gram-positive and the other is gram-negative. It is necessary to first separate the two organisms by inoculating a nutrient agar plate using the streak-plate method. The
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Investigation of the probiotic properties of bacterial strains from two probiotic drinks and their survivability in artificial gastric juice ABSTRACT: Two probiotic drinks were investigated in vitro to test their ability to survive acidic conditions and their probiotic factors. Both the products: Actimel and Yakult contain gram-positive bacteria‚ but Actimel also has a gram-negative bacteria. The ability to survive was investigated by adding artificial gastric juice to the products and incubating
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for 30 seconds
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the most people who need technology in their life‚ especially the Internet‚ today’s students are more open for the world rather than before‚ for instance‚ in the past students just used the books to do their search because their subjects were easy‚ simple and short‚ whereas‚ nowadays students are more development‚ advance and independence. Therefore‚ they need something which helps them to perform their search such as Computer with Internet because their searches are wide and include many information
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generation? a. Pasteur b. Koch c. Semmelweiss d. Lister 2. 3. 4. 5. The concept that living organisms arise from nonliving material is called a. biogenesis. b. cell theory. c. spontaneous generation. d. germ theory. Cells with a relatively simple cell morphology that do not have a true membrane-delimited nucleus are called a. prokaryotes. b. eucaryotes. c. urcaryotes. d. nocaryotes. The concept that human and animal diseases are caused by microorganisms is called the a. cell theory. b. germ
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1. Be Honest : Never try to use fake information to make others impress‚ might be fake infromation can lead your profile to look better but in this way you are lying to yourself. so be honest in whatever you are adding to it. 2. Make it Simple but a lil’ attractive: Try to make your profile simpler because simplicity is the thing that attracts most of peoples‚ but for others to reach your profile and open it you may need to make it a lil’ attractive. Making profile attractive means
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In biology 102 lab‚ I conducted an experiment dealing with worms and how they regenerate or “grow”. We did this study over a specific type of worm called Planaria‚ which are simple‚ small‚ flat worms that reside in aquatic environments and are free-living (Brewton‚ 2017‚ p. 44). To start‚ I went over to the lab counters and obtained my worm with a plastic pipette‚ sucking up the worm with a little bit of water and placing it in a tiny covered plate. The water was essential to include with the worm
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