PROTEINS AND NUCLEIC ACIDS 28/11/13 Qn. Which two of the four macromolecules do you believe to be the most important for life? Give evidence to support your suggestion (include a full description of the macromolecules you describe both in terms of structure and function) Introduction All the four macromolecules=lipids‚ proteins‚ carbohydrates and nucleic acids= are essential for the functioning of an organism ’s body (Campbell‚ biology). They work hand in hand to help us survive. For example
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e incorporated a fusion tag prior to our genes of interest and attached the expressed fusion proteins covalently on microarrays. This enhances the specific binding of the proteins compared to nitrocellulose. Thus‚ it helps to reduce the number of false positives significantly. It enables us to screen for immunogenic proteins in a shorter The correct insert size was determined by Colony PCR. Plasmids from clones containing the correct-sized inserts were isolated and the MCS was sequenced using both
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has the instructions for making proteins Part of the nucleus | function | Chromatin | * The chromatin consists of DNA and proteins. * Some of these proteins controls the activities of the cell * When cells divide‚ chromatin condenses into visible chromosomes * The chromatin shows up as dark patches when it is stained. | Nucleolus | * The nucleolus makes RNA and ribosomes-these pass into the cytoplasm and proteins are assembled on them (protein synthesis) * The nucleus is a dense
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Biology II Step 1: How will you identify the “vital” cellular protein that the virus targets for degradation? (Hint: think proteomics). (3 pts.) First‚ since we know the viral RNA sequence and its targeting protein‚ we can investigate it in bioinformatics database‚ and can acquire some clues or hints about the target proteins. From the bioinformatics database‚ we might be able to find its structure‚ similarity with other proteins‚ functions‚ and binding domains. In other words‚ we can get some
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1. PROTEIN is composed of amino acids. It is required to synthesize and repair muscle and other tissues and maintain fluid and acid-base balance in the body. Proteins also function as enzymes‚ hormones‚ and transporters. If necessary‚ the body uses protein to meet energy needs (4 kcals per gram). Excess kcals from protein are stored as body fat. If you consumed 60 grams of protein in a day‚ how many kcals would this convert to? (Choose your answer and delete the rest). (1 point) b)
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hydrogen bond between every fourth peptide bond. They are usually found in fibrous protein such as keratin and collagen. Beta pleated sheets are structures where two regions of the polypeptide chain lie parallel to each other. Again hydrogen bonds between the parts of the backbone in the parallel regions hold the structure together. The cores of many globular proteins are made of beta pleated sheets. The 3-D shape of a protein is otherwise known as the tertiary structure. Superimposed on the patterns of
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Sam Rausser Wednesday‚ August 17‚ 2011 3:38 PM Once glucose is made‚ glucose molecules can be converted (by plants) to… 1) monosaccharides‚ like fructose 2) Disaccharides a. Maltose (glu‐glu) seeds b. Sucrose (glu‐fru) sugar cane‚ sugar beets 3) Polysaccharides a. Starch‚ food storage in roots (chains of glucose) b. Cellulose‚ plant structure (chains of glucose) 4) Amino Acids a. Glucose + N from ground 5) Lipids‚ specifically oils which are energy source in seeds & phospholipids found in membranes
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This would determine the expression of a shorter protein.10 Moreover‚ the mRNA containing the premature stop codon was targeted by the nonsense mediated decay pathway which functions to decrease the expression of a potentially harmful truncated protein. Although this mechanism is hypothetical‚ it has been demonstrated that in cells derived from t(1;11) carriers DISC1 expression is reduced at both mRNA
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Bio-Rad Protein Assay INTRODUCTION Proteins are large complex organic macromolecules which are utilized for many important roles in the body. Proteins function in structure‚ transport‚ energy conversions‚ and play an important role in defense against diseases. Protein is also important for muscle recovery‚ which is why it is commonly associated with fitness. Though your body will always absorb 1g of protein the same way every time‚ protein companies like to make their product competitive
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filaments within these structures are cross-linked by accessory proteins known as actin binding proteins. These proteins connect two actin filaments through its two actin-binding sites or dimerization with actin domains. Crosslinking actin filaments can be performed by fimbrin proteins that attach filaments in a parallel fashion to facilitate movement. Thus‚ they are localized in microvilli‚ filopodia‚ and focal adhesions. Other proteins including α actinin and spectrin connect actin filaments in an
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