PNP PNP or paranitrophenol is an organic compound that undergoes changes when the pH is manipulated of the solution. PNP is also an acid/ base indicator. The compounded starts off as internal conjugated ring with the hydroxyl group and the nitro group single bonded to the aromatic ring. This occurs at the pH of 5. At this point the aromatic compound is colorless and is in the ultraviolet range. However‚ as the pH begins to increase the hydroxyl group deprotonates‚ leaving the oxygen with a negative
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Biology- Change in pH Aim: To investigate the affect that change in pH levels has on a particular enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known
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is the indicator phenolphthalein in an acidic solution and in a basic solution? (2 K/U) Phenolphthalein is colourless in acidic solutions and pinkish in basic solutions. 2) Why does the clear colour‚ which forms at the point where the hydrochloric acid comes into contact with the solution containing phenolphthalein and base in the receiving flask‚ disappear more slowly near the endpoint? (2I) At the start of the titration‚ the NaOH solution in the flask will be pinkish and the pH is quite
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* Measure respiration rate using different substrates. * Measure respiration rate at different temperature. Introduction: In this laboratory experiment‚ we are given 3 task. The first one is respiration in yeast. Second is respiratory indicator and the third one is observing mitochondria in yeast/onion cell. The first experiment is about investigation of sugars yeast. We will determine which sugars yeast can be use for cell respiration. When the yeast undergoes anaerobic/aerobic respiration
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1. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity? The product used is‚ glucose and fructose‚ this is an indicator of sucrase activity because the amount of product produced is a result of the experiment. 2. Explain why denatured sucrase was used as a control? Denatured sucrase was used as a control because it increases the activity. 3. Alkaline DNS denatures sucrase. Explain why it is important to denature the enzyme? Denaturing the
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each region to the pH results obtained. - There are four significant regions in each titration curve‚ namely the initial‚ pre-equivalence‚ equivalence‚ and post-equivalence points. These points are named according to its position relative to the equivalence point. The equivalence point signifies the volume of titrant at which the solution becomes neutral. This is represented in the graph as the drastic change in pH‚ first with a sudden increase and a sudden decrease of pH. At the initial point
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oxide. Hypothesis This experiment will be investigating the following properties of the oxides of group three; A) State at room temperature B) Appearance C) Volatility D) Conductivity of electricity of molten oxide E) Solubility in water F) pH of solution in water G) Classification of oxide (acidic‚ basic or amphoteric) H) Structure of oxide (simple molecular‚ giant molecular‚ giant ionic) I) Reactions with hydrochloric acid and/or sodium hydroxide. It is expected that some trends will
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used to neutralize the acids by using the standardized sodium hydroxide solution. It reacts with bases which is sodium hydroxide‚ NaOH to form salt and water. The equivalence point of this neutralization reaction can be determined using a chemical indicator to signal the endpoint. The graph should be plotting to take reading of the equivalence point. Once the equivalence point of the titration is known‚ the concentration of the sodium hydroxide can be determined. The sodium hydroxide is then use to
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of the chemical composition of cells is the pH of their environment. Most cells operate in a narrow pH range. That is‚ pH values above or below a certain value may cause a cell distress or may even be fatal. To help maintain relatively constant pH levels‚ living systems use buffers. A buffer is a combination of a weak acid and a weak base that function together to minimize changes in the pH of a solution. In today’s lab‚ we will learn how to measure pH and demonstrate how buffers work. II) Procedure
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crushed and weighed to the nearest 0.01 g which was 0.5003 g and 0.5014g. Then‚ transferred into a 250 mL flask and added 50.0 ml 0.1M HCl using an acid burette. Then‚ the antacid was dissolved in the acid. After that‚ the two drops of phenolphthalein indicator were added and was mixed thoroughly. Lastly‚ the antacid mixture was titrated with 0.1 M NaOH the solution turned to a faint pink color. Recorded the final volume of NaOH used. Here‚ two trials were done. After the procedures‚ the weight of HCl that
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