Post Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of β–Carotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment‚ several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by
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nCH3 The first step in the lab is the preparation of the solvent used in the developing chamber for thin layer chromatography. The solvent used is a 3:1 mixture of toluene and petroleum. After the developing chamber is prepared‚ it is essential to begin preparation of the unknown DNPH derivative[6]. The preparation of the 1‚2 DNPH derivative of a ketone is in fact a small organic synthesis which produces a fraction of a gram of product. The second part of the lab makes use of NMR Spectrometry
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depending on the number of alkyl substituents directly attached to the carbon attached to the halogen atom. The purpose of this lab was to properly prepare t-butyl chloride from t-butyl-alcohol in a concentrated hydrochloric acid. The reaction occurs through a nucleophilic substitution‚ which is when a nucleophile replaces the leaving group in the substrate. In this lab‚ the hydroxyl group of t-butyl alcohol is replaced by a chlorine atom. The reaction proceeds through an SN1 mechanism (Weldegirma
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Aim: The aim of this experiment was to determine the types of macromolecules of various foods and classify them as one or more of the following: * Carbohydrate (starch) * Lipid * Sugar (glucose) * Protein Materials: * Test tubes * Solutions : ( A‚B‚C‚D‚E‚F‚ and G) * Reagents: ( Iodine‚ Biuret‚ Benedict‚ Ethanol) * Spatula * Heating plate * A test tube cleaning brush * Tray * Beaker * Distilled water * Pipette ( comes with reagents since
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Macromolecules Biologically‚ there are four general types of macromolecules or biomolecules. They are carbohydrates‚ proteins‚ lipids and nucleic acids. Each of these plays an important role in maintaining life among living creatures and each is a piece of the elaborate puzzle of metabolism. Each has a different chemical composition and interacts with other molecules differently. Most of these biomolecules come from food sources and we are therefore already familiar with them. Without a
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Macromolecules of Life Hands-On Labs‚ Inc. Version 42-0085-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this
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Organic Chemistry Laboratory Report. Experiment 1: Crystallization. Objectives: 1. To study the crystallization process. 2. To identify the best suitable solvent to use for the crystallization process. 3. Gain an experience in purifying an organic compound by the techniques of the crystallization. Introduction. Crystallization is a technique which chemists use to purify solid compounds. It is one of the fundamental procedures each chemist
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Macro molecules A macromolecule is a very large molecule commonly created by polymerization of smaller subunits. In biochemistry‚ the term is applied to the three conventional biopolymers (nucleic acids‚ lipids‚ proteins and carbohydrates)‚ as well as non-polymeric molecules with large molecular mass such as lipids and macrocycles. The individual constituent molecules of macromolecules are called monomers(mono=single‚ meros=part). Usage The term macromolecule was coined by Nobel laureate Hermann
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Exercise 4.4: Identification of Macromolecules in Household Substances The purpose of this lab is to determine the presence of glucose‚ starch‚ and protein in various samples through standard tests. Solution 1: Formula I believe formula will test positive for sugar and protein since it is supposed to be a nutritious‚ balanced drink for infants. Materials and Methods: Please refer to the Biology 1406 Lab Manual Pg. 43 Test For | Coloration/ Appearance of Positive Control | Coloration/
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BIOLOGY LAB REPORT PRACTICAL M1 TESTING FOR MACROMOLECULES Title : Testing for Macromolecules Abstract : To test for macromolecules (carbohydrates‚ lipids‚ nucleic acids‚ and proteins)‚ iodine solution‚ alcohol‚ aceto-orcein stain and copper sulfate solution are used. The conclusions for all of the tests are positive. Introduction: A) Carbohydrate (starch) Carbohydrates are chemical compounds that contain oxygen‚ hydrogen‚ and carbon atoms. They consist of monosaccharide
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