Separation of Amino Acids by Paper Chromatography Chromatography is a common technique for separating chemical substances. The prefix “chroma‚” which suggests “color‚” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment‚ chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate and identify amino acids‚ the building blocks of proteins
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Extraction of R’-NH2 For both of the organic acid and organic base extraction‚ the addition of the respective inorganic acid or inorganic base (HCl or NaOH) to the three-component mixture caused an immiscible appearance of the solution in the separatory funnel. By briskly shaking the separatory funnel with the mixed components created a build up of pressure which was released through the stopcock forming small disappearing bubbles within the tip of the separatory funnel. Once the funnel was placed
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will show a colour change. After that record the initial reading of the burette and making sure that all your burette readings must be to an appropriate precision. Titrate the contents of the conical flask by adding to it 0.100 mole dm3 hydrochloric acid from the burette. Add the solution slowly. Swire the flask gently to mix the contents at the end indicator turns as define red colour. This is the end point of the titration. Record the final burette reading in your table of result. Repeat the titration
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Essay March 28‚ 2014 ACID RAIN ENVIRONMENAL EFFECTS What is acid rain? It’s a rain or any other form of precipitation that is unusually acidic‚ meaning that it possesses elevated levels of hydrogen ions (low pH). It can have harmful effects on plants‚ aquatic animals and infrastructure. Acid rain is caused by emissions of sulfur dioxide and nitrogen oxide‚ which react with the water molecules in the atmosphere to produce acids. The most obvious environmental effect of acid rain has been the loss
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Acid in the human stomach When people refer to acid they usually see it as a corrosive liquid that can dissolve metals or change the color of a substance. So they think of acid as a dangerous matter for the body and believe it can easily dissolve your skin. What I have previously listed are indeed properties of certain acids‚ such as Hydrofluoric Acid which can cause irreversible tissue damage if it comes in contact with skin‚ but not all acids are this strong. As a matter of fact what a lot of
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PRODUCTION OF CITRIC ACID Introduction: Citric acid is a White solid‚C3H4OH(COOH)3‚soluble in water and slightly soluble in organic solvents‚ which melts at 153 oC. Aqueous solutions of citric acid are slightly more acidic than solutions of acetic acid. Traces of citric acid are found in numerous plants and animals‚ because it is a nearly universal intermediate product of metabolism. Large amount of the acid are found in the juice of citrus fruits‚ from which it is precipitated by the
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Acids and Bases Shane Purpose- To study the relationship between pH and metabolism. 10/21/13 Materials- see attached Per. D Biology Procedure- see attached Data and Results- see attached Theory- PH stands for the power of hydrogen. People refer to the pH scale as a tool for finding out if a substance is acidic‚ basic‚ or neutral. This scale goes from zero to fourteen. The number seven is neutral. Any number higher than seven on this scale is basic. Any number lower is acidic
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expected melting point. After its temperature reached the plateau‚ the temperature increment was set at 1°C per minute‚ and the melting point range of 152°C-154°C was recorded. After standardizing the melting device‚ a small sample of unknown carboxylic acid was placed in a capillary tube‚ and an arbitrary plateau of 200°C was set. When the sample started to melt at 150°C‚ another capillary tube containing a small amount of unknown sample was placed in the melting device‚ and the plateau was set to 135°C
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Acids And BAses Acids And BAses 8.1 8.2 8.3 8.4 18.1 18.2 18.3 18.4 18.5 Theories of acids and bases Properties of acids and bases Strong and weak acids & bases The pH scale Calculations involving acids and bases (AHL) Buffer solutions (AHL) Salt hydrolysis (AHL) Acid-base titrations (AHL) Indicators (AHL) 8 8.1 THeORies OF Acids And BAses 8.1.1 Define acids and bases according to the Brønsted–Lowry and Lewis theories. 8.1.2 Deduce whether or not a species could act as a Brønsted–Lowry
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Experiment #5: Acid Burn!!! Introduction The goal of this lab was to correctly prepare a 0.2M solution of NaOH‚ identify highly acidic household cleaning chemicals‚ and determine their concentration (molarity) through titrations using the previously prepared 0.2M NaOH solution. Experimental First‚ to create 0.5L of 0.2M solution of NaOH‚ standard 3M NaOH solution was obtained. Next‚ calculations were performed to determine the amount 3M NaOH necessary to create 0.5L of the 0.2M solution and‚ as
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