1) Why serial dilutions are preferred to one large dilution in lab experiments? Answer: Serial dilution is the technique of performing repeated dilutions on the same chemical in order to change its concentration. The diluted solution from a serial dilution can be used to calculate the concentration of the actual solution. In experimental work‚ often we need to obtain a range of concentrations for a specific compound. Thus‚ instead of preparing one large dilution‚ if we take a concentrated sample
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Vocabulary: Lesson pH and dilutions Science Starter: Determine the pH of .0103M H3PO4 solution in water. Remember to look at the H+ count. Box 1 In a dilution‚ the number of solute particles does not change‚ just the concentration with respect to the volume. This relationship is _____________________ proportional. Initially‚ there is 275mL 0.125M stock solution of a substance. -Determine the amount of moles of solute present. -What is the concentration after a dilution when the final volume is
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DILUTIONS AND STANDARDS Many of the laboratory procedures involve the use of dilutions. It is important to understand the concept of dilutions‚ since they are a hand tool used throughout all areas of the clinical laboratory. These dilutions have to be considered as they make a quantitative difference in what is going on. First‚ there are several terms used in expressing dilution: 1. "Dilution: - Dilutions are expressed as the ratio of the quantity of a desired solute (serum‚ urine‚ chemical solution
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concentration of the coloured complex can be determined with the spectrophotometer at Absorbance 540.The sugar concentration of unknown sample can then be read off a calibration curve(standard curve) created using known sugar concentrations.The dilutions of a solution of known concentration are used to determine the concentration of unknown. Being familiar with the background information about reducing sugars and various methods used to identify them‚Biotechnology students were provided with a
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concentration (in ppm) of phosphate can be controlled. First‚ six samples of varying phosphate concentration (0.5‚ 1.0‚ 2.0‚ 3.0‚ 4.0‚ 5.0 ppm) will be made by diluting 20 ppm stock phosphate solution with deionized water. This can be achieved by using the dilution equation: M1V1=M2V2. Due to phosphate being uncolored an ammonium molybdate will be used to react with phosphate in the water to form a phosphomolybdate complex. This complex will change color when reacted with a Sn(II)Cl2 solution turning it a dark
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Their preference for these sweeteners vary depending on their concentration. To understand the specific concentration of sucrose preferred by Drosophila‚ we exposed them to a serial dilution of sucrose. After obtaining the concentration of sucrose that is most preferred‚ we created a serial dilution of quinine with this concentration to examine whether Drosophila could detect different concentrations of quinine. Since aging in humans is accompanied with a gradual loss of physiological functions
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cuvettes‚ 5mL volumetric pipettes‚ droppers‚ 1mL volumetric pipettes‚ safety goggles‚ rubber gloves‚ and aprons. Procedure Label six test tubes (1 through 5 and B for blank). A series of 5 test solutions of Ferric Chloride are made by serial dilution. Dilute 1Ml of the stock Ferric Chloride to 10 mL with de-ionized water to make a 0.01M solution. Place 1mL of the 0.01M Ferric Chloride solution in the first test tube
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solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample‚ and determine through observations whether or not addition of galactose or mannose will inhibit this reaction. I hypothesize that the Con A + galactose solutions will have partial agglutination
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Title: Counting the Number of Yeast Cells in a Suspension using Haemocytometer Objective: To estimate the number of cells of yeast per mm3 in five different dilutions of yeast suspension. Introduction: Biologists often need to count the density of cells in a liquid. “Density of cells” means “the number of cells per unit volume of liquid”. For example‚ they might want to find out the density of red blood cells in blood plasma‚ the density of bacteria in milk‚ or the population of Paramecium sp
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make the standard curve was made by serial dilutions. Human control DNA with known concentration was used: 1. First was diluted 1 to 11 and the concentration was 1ug in 5ul 2. 1ul of previous dilution in 9ul H2O > 0.01ug DNA in 5ul 3. 1ul of previous dilution in 9ul H2O > 0.001ug DNA in 5ul 4. 1ul of previous dilution in 9ul H2O > 0.0001ug DNA in 5ul 5. 1ul of previous dilution in 9ul H2O > 0.00001ug DNA in 5ul To make my DNA sample I used dilutions: 1. 1 to 10 2. 1 to 100 The master
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